Exam II

Question 0

Papain enz

Answer 0

2 Fab: binds Ag

Fc: signals immune fn

Question 1

Region of Ab binds Ag

Answer 1

V-region w/ 3 CDRs

Fc region binds Fc-R on phagocytes

Question 2

Pepsin enz

Answer 2

F(ab’)2: 1 fragment w/2 binding sites

Question 3

IgG

Answer 3

Monomer
3 C-domains

Opsonize, humoral imm, NK Cells, neonate imm

Long t 1/2

Question 4

IgA

Answer 4

Mono/di/tri- prime dimer

Aerosol & mucosa

Oral polio vax

Question 5

IgM

Answer 5

Pentamer/hexamer

Prime BCR (naïve): B1(CD5) & B2 marginal cell
❇️C'

Question 6

IgE

Answer 6

Monomer

4 C-domains

⛔️parasites & allergies➡️✳️mast cell degranulation

Question 7

IgD

Answer 7

Other naïve BCR (follicular B2:IgM & D)

Unk fn

Question 8

Immunoglobulin chains

Answer 8

HC: isotype class fn

LC: k & L but same fn

Question 9

HSC(CD43)➡️CLP➡️TF: EBF/E2A/Pax5➡️

Answer 9

Pro-B➡️Il-7:✳️ expansion + gene rearr

✅HC preBCR➡️ pre-B
✅LC➡️ IgM: immature B-cell
❇️/⛔️selection

Question 10

HSC➡️CLP➡️TF:Notch-1/GATA-3➡️

Answer 10

Pro-T➡️Il-7:❇️ exp + rearrange

Question 11

[Ig & TCR-ß] HC locus

Answer 11

D+J➡️ V+DJ➡️VDJ+C

Question 12

LC Locus

Answer 12

⛔️D-region

C: k or L

VJC

Question 13

T-cell dep Ag

Answer 13

Fol B2-cell: Req CD4 recog SAME PROTEIN Ag

✳️HC class switch
⬆️aff maturation 
✳️long live Plasma cells

Question 14

T-cell indep Ag

Answer 14

Marginal B2-cell & B1-Cell
⛔️CD4 help

Polysacc/lipids/nuc acid

❇️short lived Plasma cells: only low aff IgM secr

If cytokines: class switch

Question 15

1st Exposure: ALL immunogens

Answer 15

Naïve B-cell➡️Plasma cells: ❇️short lived, low level Ab prod IgM>IgG

Question 16

2nd exposure: PROTEIN Ag only

Answer 16

Rapid Memory cell➡️Plasma cell

1. Class switch: ⬆️IgG secr
2. ⬆️aff maturation

Question 17

(2) BCR Complex+ Ag

Answer 17

IgM + Iga&Igß➡️ x-link:✳️ITAM-P➡️
Syk⏩P-cascade⏩
❇️TF: NFAT+ NFk-ß + AP-1

Question 18

Ab fn

Answer 18

NOAC

Neutralize
Opsonize
ADCC➡️NK Cells: Fc:Fc-R
Classic C’

Question 19

Vax IgG

Answer 19

Tetanus: toxiods
Hep A&B: env protein
Pneumonia: polysacc&protein (&IgM)

Question 20

IgG+Ag➡️❇️Fc➰R1

Answer 20

❇️phagocytosis➡️kill

Question 21

IgG+Ag➡️❇️Fc➰RIII

Answer 21

Low aff:✳️NK Cell➡️ADCC kill

Question 22

Worm+ IgE➡️❇️Fc€R1

Answer 22

✳️eosinophil➡️❇️rel granules➡️✳️kill

Question 23

Classic C’ path

Answer 23

Req (2)IgG/ IgM pentamer➡️ recruit C1
C1+Fc of Ab:✂️C4&C2 ➡️C3 convertase➡️ ✂️C3+C5➡️

❇️C5 convertase➡️C678⏩ C9 MAC Pore➡️cell lysis

Question 24

Alt C’ path

Answer 24

⛔️Ab

C’ proteins bind directly on pathogen surface

C3+ FactorD✂️➡️C3 convertase⏩MAC pore

Question 25

Lectin C’ path

Answer 25

⛔️Ab

Plasma Lectin bind Mannose-R on pathogen surface➡️✳️C4&C2 ➡️C3 convertase➡️ ✂️C3+C5➡️

❇️C5 convertase➡️C678⏩ C9 MAC Pore➡️cell lysis

Question 26

T- cell dev tolerance

Answer 26

Central: ⛔️select➡️apop or reg T-cell
Periph: req MHC:TCR & B7:CD28 sig

⛔️B7 or B7:CTLA4➡️ ✳️anergy

Question 27

Reg T-cell

Answer 27

Contact dep inhib
Contact dep inhib: cytokines

Il-10: ⛔️APC fn & ⛔️M➰ activation
TGF-ß: ⛔️T-cell prolif & ⛔️M➰ activ

Question 28

Factors for Tolerogen vs Immunogen

Answer 28

Amt
Duration
Entry
Adjuvant
APC costim molecules

Question 29

B-cell dev tolerance

Answer 29

Central: low avid➡️ anergy + ⬇️R exp
High avid➡️ apoptosis or R edit

Periph: regu by inhib-R,
⛔️CD4 help➡️anergy or apoptosis

Question 30

CD4:B-cell late response

Answer 30

Germinal center:Dark zone➡️light zone

IFN-➰: IgG2a
Il-4: IgE & IgG1

⛔️cytokine: ⛔️class switch➡️secr IgM