Exam 3: Antifungals Flashcards
What is the MOA of antifungals and what are the two exceptions to this?
They target the cell membrane or cell wall, except for Griseofulvin and Flucytosine
What is Amphotericin B produced by?
Streptomyces Nodosus
What is the MOA of amphotericin B?
Interaction with sterol of fungal membrane, ergosterol, that results in the loss of intracellular components through depolarization and pore formation
What is the spectrum of Amphotericin B. Is it fungicidal or static?
Broad spectrum.
Fungicidal
What is the DOC for most systemic fungal infections?
Amphotericin B
What is the likely cause of the toxicities of amphotericin?
It binds to human membrane sterols as well as fungal sterols.
Due to either
1) Infusion of drug
2) Reactions occurring over time
What are the infusion related toxicities of Amphotericin B?
Immediate.
Chills, fever, muscle spasms, headache, vomiting. May occur with each injection and can be lessened by slowing the infusion rate or decreasing daily dose
What are the toxicities that occur from reactions over time with Amphotericin B?
- Powerful nephrotoxicity agent (dose dependent and can cause irreversible kidney damage)
- Azotemia (BUN and creatinine elevated)
- Hepatic failure
- Hypersensitivity
What is the MOA of Flucytosine?
Flucytosine is converted to 5- fluorouracil which interferes with fungal DNA and RNA synthesis
What is the spectrum of Flucytosine?
Lower than amphotericin B, used for cryptococcus neoformans, some strains of candida, aspergillosis fumigatus, etc.
What is the DOC for crytococcus infections?
Flucytosine plus amphotericin B (significant synergistic effect)
Does Amphotericin B or Flucytosine have better CSF penetration?
Flucytosine
What are the toxicities of Flucytosine?
Depression of bone marrow, GI disturbace, elevated ALT/AST
What is the MOA of the “-azoles”?
Static or cidal?
Inhibits synthesis of ergosterol, leads to depletion of ergosterol and accumulation of toxic sterols causing increased permeability and inhibition of fungal growth.
Fungistatic
What is the spectrum of ketoconazole?
Broad spectrum antifungals
Poor CNS penetration
What are the toxicities associated with ketoconazole?
- Potent inhibitor of P450s, drug interactions
- Gynecomastia and impotence due to inhibition of adrenal and testicular function
- Prolonged QT
What are the contraindications of Ketoconazole?
Hepatic disease
Why is fluconazole used in Fungal meningitis?
It penetrates well into body fluids, particularly CSF where drug concentration reaches 50-90%