Exam 2 week 2 Flashcards
Which viruses depend on host DNA dependent DNA pol
Parvo papilloma polyoma
define eclipse period
time from infection to detection of new virus
define latent period
time from infection to detection of EXTRACELLULAR virus
burst size
amount of new virus from a single infected cell
what are the receptors for HIV virus
Primary- CD4, secondary= CCR5, CXCR4
EBV receptors are
primary CD21, secondary MHCII
Influenza receptors are
terminal salic acid on glycolipids or glycoproteins
rhinovirus receptors
ICAM1
rabies virus receptors
nACHr, NCAM
measles receptors
CD46, CD150
what is tropism
the kind of cells a virus can infect depending on the host cell receptor expression
How do enveloped viruses enter cells
1) viral lipid membrane merges with PM of host cell and releases nucleocapsid into the cytoplasm.
2) endocytosis of virus, low pH of endosome allows fusion to the endosomal membrane and release of nucleocapsid to the cytoplasm
how do naked viruses enter cells
by endocytosis. the low pH of endosome changes the capsid and release of material to cytoplasm
what is uncoating
the release of genetic material from the nucleocapsid. Reo is loose capsid
how does herpes reproduce
enters cells and uses RNA poll to make TF and DNA pol
how does polyoma and papilloma replicate
they do not have or encode viral DNA pol. they need to activate the cell in order to get viral DNA replicated
Which viruses uses their own pol to copy genetic material. why is it important
Adeno, herpes, pox, hepadna have own DNA pol. The DNA pol is a great target of antiviral therapy
What viruses do not have own virally encoded pol? How do they replicate
Papillomo, polyoma and parvo. Papilloma and polymer kicks the cells into S phase to replicate. Parvo must enter actively dividing cells.
What is the replication for Pox viruses like
Exception: DNA virus that carries their own RNA Pol in the virion can replicate in the cytoplasm. Have a complex envelope
What is notable about HBV/hepadna
HBV is replicated in both the nucleus and the cytoplasm. It replicates DNA genome through RNA intermediate via own reverse transcriptase. NTRIs can be used in therapy
What is notable about herpes virsus
it can enter latent period for years like shingles and cold sores
How do +ssRNA replicate
They can be made directly into mRNA. They make polyproteins that need to be cleaved by viral proteases (all +ssRNA viruses have viral proteases) and viral RNA dependent RNA pol is made. The viral RNA pol can make both mRNA and -ssRNA.
GOOD TARGET FOR THERAPY IS PROTEASE OR POLYMERASE
What type of pol do +ssRNA viruses use
they encode (don’t bring in) their own RNA dependent RNA pol.
How do retroviruses replicate
Retroviruses- HIV and HTLV convert +ssRNA into 2 copies to make DNA (called diploid). Proviral DNA integrates into the host cell DNA via integrate and remains for the life of the cell.