Exam 2: Major Proteins in DNA Replication Flashcards
1
Q
helicase
A
moves along the fork unwinding dsDNA into two strands of ssDNA
2
Q
SSBP
A
single-strand binding protein
keeps ssDNA strands apart
3
Q
topoisomerases
A
type 1 and 2
eliminate excess supercoiling generated by unwinding
4
Q
topoisomerase 1
A
nick, rotate, ligate
5
Q
topoisomerase 2
A
double-strand break, pass through, rejoin
6
Q
primase
A
makes short RNA primers on ssDNA strand
7
Q
DNA polymerase 1
A
rips our RNA primer, puts in DNA, in steps of one nucleotide at a time
8
Q
DNA polymerase 3
A
5’3’ polymerase
synthesizes DNA starting at an RNA primer
9
Q
ligase
A
closes nicks in the sugar-phosphate backbone of one ssDNA strand in dsDNA
10
Q
telomerase
A
enzyme that restores full-length telomeres in the chromosomes of gametes
renews telomeres in stem cells when they divide
