Exam 2 Flashcards
When would you use the most probable number method? a) when the organism is resistant to antibiotics b) when the organism cannot grow on agar c) when the organism is sensitive to antibiotics d) when you are counting the organism with a coulter counter
b) when the organism cannot grow on agar
Bacteria reproduce by the process called: a) meiosis b) spontaneous generation c) mitosis d) spontaneous mutation e) binary fission
e) binary fission
Cells may enter the stationary phase of growth because of: a) depletion of an essential nutrient b) lack of available oxygen c) the accumulation of toxic waste d) all of the above
d) all of the above
An inhibitor of nucleotide biosynthesis is: a) penicillin b) streptomycin c) 6 mercaptopurine d) para amino benzoic acid e) uridine diphosphate
c) 6 mercaptopurine
ATP synthesis during fermentation occurs by: a) substrate level phosphorylation b) Krebs cycle c) oxidative phosphorylation d) adenitization e) electron transport
a) substrate level phosphorylation
You are working on a study of the viable cells in a bacterial suspension. You would count these cells using: a) Petroff Hauser cell b) coulter counter c) spread plate method d) both a and b
c) spread plate method
Electron transport chains are located in: a) ribosomes b) membranes c) cell walls d) periplasmic space e) glycolayx
b) membranes
Aerobic respiration involves: a) glycolysis b) The Krebs cycle c) the electron transport system d) all of the above
d) all of the above
Aspartate + a-ketoglutarate —> oxaloacetate + glutamate This is an example of a: a) transamination b) deamination c) oxidation d) decarboxylation e) dehydrogenation
a) transamination
Essentially the second law of thermodynamics says that: a) energy can neither be created nor destroyed b) entropy is increasing in the universe c) one form of energy can not be converted into another d) energy can not be converted into matter or matter into energy
b) entropy is increasing in the universe
Entropy is a measure of: a) increase in orderliness b) gain of high-level energy c) increase in potential energy d) increase in kinetic energy e) increase in randomness
e) increase in randomness
What bacterium is associated with gas gangrene? a) Pseudomonas aeruginosa b) Escherichia coli c) Clostridium perfringens d) Methanococcus jannaschii e) phospholipase
c) Clostridium perfringens
The breakdown of larger more complex molecules into smaller simpler molecules with the release of energy and the trapping of some of that energy: a) anabolism b) catabolism c) Pasteur effect d) electron transport e) proton motive force
b) catabolism
ATP is the energy molecule in cells because: a) it is big b) it carries a charge c) it contains a high energy phosphate bond d) it is made of glucose
c) it contains a high energy phosphate bond
The energy required to bring the reacting molecules together in the proper way to reach the transition state is called the: a) activation energy b) free energy c) entropy d) enthalpy e) calorie
a) activation energy
NADP is reduced to NADPH in the: a) glycolytic pathway b) pentose phosphate pathway c) glycoxalate cycle d) Entner Douderoff pathway e) Kreb’s cycle
b) Pentose phosphate pathway
The precursor of GMP and AMP: a) IMP b) orotic acid c) UMP d) tetrahydrofolic acid e) methotrexate
a) IMP
Which of the following is an example of an electron carrier molecule? a) citric acid b) CO2 c) acetyl CoA d) NAD e) ATP
d) NAD
An example of an iron sulfur protein: a) cytochrome b) ubiquinone c) isoprenoid d) flavoprotein e) ferrodoxin
e) ferrodoxin
Coenzyme A plays an important role in the synthesis of: a) amino acids b) orotic acid c) proteins d) lipids e) nucleotides
d) lipids
The chemical reaction that involves the gain of elctrons is called: a) condensation b) oxidation c) hydrolysis d) ionization e) reduction
e) reduction
The enzyme that breaks down starch is called: a) caseinase b) lipase c) starchase d) amylase e) cellulase
d) amylase
What is the name of the process by which reduced NADH transfers electrons along a chain of acceptors to oxygen so as to form water and in which the enrgy released along the way is used to form ATP: a) glycolysis b) acetyl CoA formation c) Krebs cycle d) electron transport phosphorylation
d) electron transport phosphorylation
A type of chemical reaction that requires energy be supplied from the surrounding environment: a) exergonic b) endergonic c) condensation d) hydrolysis
b) endergonic
Electron transport chains are located in: a) ribosomes b) membranes c) cell walls d) periplasmic space e) glycolayx
b) membranes
Glucose + glucose —-> maltose + water This is an example of a: a) peptide bond formation b) hydrolysis c) dehydration synthesis d) phosphodiester bond formation
c) dehydration synthesis
Name an organism that can convert fumarate to succinate using anaerobic respiration: a) Streptococcus sp. b) Pseudomonas aeruginosa c) Clostridium perfringens d) Methanococcus jannaschii
a) Streptococcus sp.
A pure culture: a) is sterile b) has only one type of organism growing on it c) is made up of clearly defined chemicals d) is pathogenic
b) has only one type of organism growing on it
You are working for Jack Daniels distillery and want to make ethanol form glucose. The organism that you would use for this process is: a) pneumocystis carinii b) Methanococcus janaschii c) Escherichia coli d) Chlamydomonas sp. e) Saccharomyces cerevisiae
e) Saccharomyces cerevisiae
Name a bacteria that undergoes a lactic acid fermentation: a) Serratia marcescens b) Escherichia coli c) Lactobacillus bulgaricus d) Enterobacter aerogenes e) Pseudomonas aeruginosa
c) Lactobacillus bulgaricus
An example of an iron sulfur protein: a) cytochrome b) ubiquinone c) isoprenoid d) flavoprotein e) ferrodoxin
e) ferrodoxin
An example of a bacteria that undergoes a mixed acid fermentation is: a) Serratia Marcescens b) Escherichia coli c) Lactobacillus bulgaricus d) Enterobacter aerogenes e) Pseudomonas aeruginosa
b) Escherichia coli
The chemical reaction that involves the loss of electrons is called: a) condensation b) oxidation c) hydrolysis d) ionization e) reduction
b) oxidation
Name an amylase producing organism: a) Actinomyces sp. b) Bacillus subtilis c) Mycobacterium sp. d) Neisseria meningitidis e) Escherichia coli
b) Bacillus subtilis
Name an organism that can convert H2SO4 to H2S: a) Bacillus subtilis b) Thiobacillus thiooxidans c) Rhodospirillum sp. d) Lactobacillus acidophilus e) Desulfovibrio sp.
e) Desulfovibrio sp.
An organism that uses CO2 —-> CH4 as the final electron acceptor in anaerobic respiration: a) Pseudomonas aeruginosa b) Escherichia coli c) Clostridium perfringens d) Methanococcus jannaschii
d) Methanococcus jannaschii
Enzymes: a) control the speed of a reaction b) changes shape to facilitate certain reactions c) must be present for any organic chemical reaction to occur d) all of the above e) a and b
e) a and b
NAD is: a) a coenzyme b) a hydrogen carrier c) an oxygen carrier d) both a and b are true
d) both a and b are true
The reacting molecules in an enzyme catalyzed reaction are called: a) entropy b) oxidants c) products d) substrates e) inhibitors
d) substrates
The product of gluconeogenesis is: a) acetic acid b) pyruvic acid c) glucose d) lactic acid e) butanediol
c) glucose
A medium that distinguishes among different groups of bacteria on the basis of their growth characteristics is called a: a) selective media b) all purpose media c) differential media d) all of the above e) both b and c
a) selective media
Name a cellulase-producing organism: a) Actinomyces spp. b) Bacillus subtilis c) Mycobacterium spp. d) Niesseria meningitidis e) Escherichia coli
a) Actinomyces spp.
The final acceptor for hydrogen ions in aerobic respiration is: a) oxygen b) pyruvic acid c) PGAL d) glucose e) CO2
a) oxygen
The products of the Emden Meyerhoff pathway are: a) carbon dioxide and water b) lactate and oxidized NAD c) pyruvate and acetate d) two pyruvates e) pyruvate and glyceraldehyde 3-PO4
d) two pyruvates
During the lag phase of microbial growth the organisms are metabolically inactive: a) true b) false
b) false
The enzyme lipase converts triglycerides into: a) glycogen b) glycerol and fatty acids c) glucose d) pyruvic acid e) glucose and galactose
b) glycerol and fatty acids
A medium that contains ingredients of a precise chemical composition is called a: a) selective media b) complex media c) differential media d) defined media
d) defined media
A nitrogenous base containing a single ring: a) pyrmidine b) purine c) ribose d) deoxyribose
a) pyrmidine
An organism that contains Beta-galactosidase is: a) Shigella sonnei b) Salmonella typhimurium c) Escherichia coli d) Proteus vulgaris
c) Escherichia coli
The energy required to bring the reacting molecules together in the proper way to reach the transition state is called the: a) activation energy b) free energy c) entropy d) enthalpy e) calorie
a) activation energy
