Exam 2 Flashcards
1. Which of the following enzymes or cofactors does not participate in redox reactions in living organisms? A. Dehydrogenase B. Oxidase C. Kinase D. Oxygenase E. Cytochrome
C. Kinase
A kinase is a transferase (enzyme which transfers one functional group to another) of phosphate groups. No redox reaction (change in the oxidation state of the substrate) happens since the reactant’s amino or hydroxyl group is exchanged for the phosphate group.
Which of the following reactions results from the flow of protons down a proton gradient? A. ADP + Pi -> ATP + H2O B. Glycolysis C. Gluconeogenesis D. Amino Acids Protein E. Glucose + Fructose Sucrose
A. ADP + Pi -> ATP + H2O
Which of the following is amphibolic? A. Electron transport chain B. Gluconeogenesis C. Glycolysis D. Citric Acid Cycle
D. Citric Acid Cycle
The term amphibolic is used to describe a biochemical pathway that involves both catabolism and anabolism. This term was proposed by B.Davis in 1961 to emphasise the dual metabolic role of such pathway. The citric acid cycle (The Krebs Cycle) is a good example of amphibolic pathway.
Catabolism : Anabolism :: ____ : ____
A. Exergonic; Endergonic
B. Work; Energy
C. Entropy; Enthalpy
A. Exergonic; Endergonic
The step involved in the isomerization of an aldose to a ketose: A. Phosphorylation of glucose B. Conversion of G6P to F6P C. Dehydrogenation of G3P D. Formation of Phosphate
B. Conversion of G6P to F6P
Glycolysis Intermediate | Compound Type
Glucose | Aldehyde
Glucose-6-Phosphate | Aldehyde
Fructose-6-Phosphate | Ketone
Fructose-1,6-bisphosphate | Ketone
Dihydroxyacetone phosphate | Ketone
Glyceraldehyde-3-phosphate | Aldehyde
1,3-bisphosphoglycerate | Carboxylic Acid
3-phosphoglycerate | Carboxylic Acid
2-phosphoglycerate | Carboxylic Acid
Phosphoenolpyruvate | Carboxylic Acid
Pyruvate | Carboxylic Acid
Choice A: Glucose -> Glucose-6-phosphate
Choice B: Glucose-6-phosphate -> Fructose-6-Phosphate
Choice C: Specifically refers to glyceraldehyde-3-phospate -> 3-phosphoglycerate (carboxylic acid) but the same enzyme catalyzes the phosphorylation to 1,3-bisphosphoglycerate
Choice D: The reactant was not mentioned, so we cannot determine what kind of compound it is.
Substrate level phosphorylation A. Glucose -> G6P B. G6P -> F6P C. Glyceraldehyde-3-Phosphate -> 1,3-bisphosphate D. Phosphoenol Pyruvate -> Pyruvate
D. Phosphoenol Pyruvate -> Pyruvate ?
Which of the following inhibitors of glycolysis will cause the greatest loss in energy yield?
a. 2-Deoxyglucose
b. Sulfhydryl Reagents
c. Fluoride
d. Arsenate
d. Arsenate
Arsenate- looks like Pi; able to substitute for Pi in enz-catalyzed rxns; glycolysis continues unabated but net ATP synthesis does not occur
Which statement about gluconeogenesis is not true?
a. The rate limiting step is the provision of carbon skeleton by muscle amino acids
b. Propinoyl-CoA is a minor source of substrates
c. G6P & Pyruvate Carboxylase can only be found in gluconeogenesis
d. Acetyl-CoA is the major source of carbon skeletons for gluconeogenesis
d. Acetyl-CoA is the major source of carbon skeletons for gluconeogenesis
Propionyl CoA can serve as a minor precursor for gluconeogenesis
Which apolipoprotein is involved in the transfer of cholesterol esters in reverse cholesterol transport?
a. Apo A1
b. Apo A2
c. Apo C1
d. Apo C2
a. Apo A1
Which of the following does not increase fatty acid synthesis?
a. Increased CHO consumption
b. Increased fat consumption
c. Increased ethanol consumption
d. High Glucagon level
d. High Glucagon level
What is the most common form of bile salt?
a. Cholic Acid
b. Chenodeoxycholic Acid
c. Deoxycholic Acid
d. Lithocholic Acid
Cholanoic acid?
Which of the following is not directly converted to Pyr or TCA molecules?
a. Asp
b. Glu
c. Val
d. Ala
c. Val
Note the keyword here is directly. All of these molecules are glucogenic (converted to pyruvate or TCA intermediates) but only valine needs more than one reaction to become the TCA product Succinyl-CoA
The rest (Asp, Glu, Ala) are directly (through only one reaction) converted to pyruvate/TCA intermediates through deamination.
What is the classification of the amino acid that yields only Acetoacetyl CoA/Acetyl CoA during the catabolism of its carbon skeleton?
a. Glycogenic
b. Ketogenic
c. Glycogenic and Ketogenic
d. Essential
e. Non-essential
b. Ketogenic
Glycogenic - if the carbon skeleton of the amino acid can be used to provide net synthesis of glucose.
Ketogenic - if no carbons from the amino acid can provide net synthesis of glucose, but instead yield acetoacetate and acetyl CoA (Leu, Lys)
Why is aspargine considered a semi-essential amino acid?
- Sure ba itong tanong na ito? Non-essential kasi ang asparagine.
Anyway, the semi-essential amino acid Arginine may be synthesized in the body from Glutamate but the Arginine formed via this pathway is insufficient to meet requirements for growth, making it semi-essential.
Considering the interrelationship between the 3 carriers of 1 carbon group, which compound will accumulate in the absence of Vit B12?
a. N5-methyl TH4
b. Homocysteine
c. SAM
d. Methylcobalamine
b. Homocysteine
Phenylalanine deficiency manifests as neurological disorders. Which of the following neurotransmitters are affected in phenylalanine deficiency?
a. Norepinephrine
b. Epinephrine
c. Dopamine
d. AOTA
d. AOTA
Which of the following pairs of compounds is utilized to transport ammonia produced in muscles and other peripheral tissues to the liver?
a. Glutamate and Alanine
b. Alanine and Glutamine
c. Glutamate an Glutamine
d. Pyruvate and Urea
b. Alanine and Glutamine
Transport of Ammonia to the liver as Glutamine (from peripheral tissues) via Glucose alanine cycle (from muscle)
What enzyme catalyzes the regulating step of fatty acid oxidation? A. Acyl CoA Synthetase B. Carmitine Acyltransferase I C. Carmitine Acylcarmitine Translocase D. Carmitine Acyltransferase II
B. Carmitine Acyltransferase I
Malonyl-CoA is a powerful inhibitor of the enzyme carnitine acyltransferase I, which starts the process of oxidation by transporting fatty acids into the mitochondrion
How many ATPs can be produced from the complete oxidation of Lauric acid (C=12)?
a) 68
b) 78
c) 88
d) 108
b) 78
After initial activation (-2 ATP), five beta-oxidations (5x4 ATP = +20 ATP) will change lauric acid into 6 acetyl-SCoA molecules (6x10 ATP = +60 ATP). The tota; energy yield is 78 ATP per lauric acid.
What is the rate-limiting step in the synthesis of cholesterol?
a. Synthesis of mevalonate
b. Mevalonate to isoprenes
c. Isoprenes to squalenes
d. Squalenes to Lanosterol
a. Synthesis of mevalonate
The rate-limiting step in the pathway to cholesterol (and
a major site of regulation) is the conversion of HMGCoA
to mevalonate (Fig. 21–34), the reaction catalyzed
by HMG-CoA reductase. (Lehninger)
Which intermediate initiates fatty acid synthesis?
a) Acetyl-CoA
b) Acetoacetyl CoA
c) HMG CoA
d) Malonyl CoA
d) Malonyl CoA
A three carbon intermediate, malonyl-CoA, initiates fatty acid synthesis
One enzyme regulated by AMPK is acetyl-CoA carboxylase, which produces malonyl-CoA, the first intermediate committed to fatty acid synthesis. Malonyl-CoA is a powerful inhibitor of the enzyme carnitine acyltransferase I, which starts the process of oxidation by transporting fatty acids into the mitochondrion. (Lehninger)
First reaction in fatty acid synthesis
a. Condensation
b. Dehydrogenation
c. Reduction
d. Dehydration
a. Condensation
Condensation - The first reaction in the formation
of a fatty acid chain is condensation of the activated
acetyl and malonyl groups to form acetoacetyl-ACP,
an acetoacetyl group bound to ACP through the phosphopantetheine OSH group.