Exam 2 Flashcards
!!!!! Definitions
Reactions covalently link monomers together to form polymers are called _______, where as reactions that break polymers into monomers are _____ reactions.
Dehydration
Hydrolysis
Functional groups:
NH2
PO4
CH3
NH2 amino group
PO4 phosphate group
CH3 methyl group (non polar)
!!!!!! 2 storage polysaccharides
-starch
-glycogen
-cellulose
(All polymers of glucose)
3 general classes of lipids:
- triglycerides
- phospholipids
- steroid lipids
3 functions of proteins:
- energy storage
- structure
- generate movement (motor proteins)
!!!!! 2 types of bonds/attractions that hold side chains
(R groups) of amino acids together to contribute to tertiary structure of polypeptide:
- hydrogen bonds
- disulfide bridges
3 components of a nucleotide of a DNA:
- 5-carbon sugar (pentose)
- nitrogenous base
- phosphate group
Names of the monomers that make up these polymers:
nucleic acids
Polypeptides
Polysaccharides
Nucleotides
Amino acids
Monosaccharide
2 ways nucleotide in DNA differs from nucleotide in a RNA
- sugar present
- uracil and thymine
Functional groups on 2 ends of polynucleotide
chain
Hydroxyll 3’
Phosphate 5’
!!!! DNA polymerase I
Removes nucleotides from RNA primer.
!!! Topoisomerase
Prevents over winding
!!!!! DNA polymerase II
Adds nucleotides to leading strand of DNA
!!!! Okazaki fragment
Has a primer and segment of DNA on lagging strand of DNA
!!!!! Ligase
Makes final phosphodiester bond between 2 Okazaki fragments
!!!!! Primase
Lays down short sequence of RNA nucleotides before DNA Polymerase III can begin replication
!!!! Helicase
Breaks hydrogen bonds between complimentary bases at replication fork
!!!!! Origin
Sequence of DNA nucleotides where replication begins
!!!!!! Single-stranded binding proteins
Binds to nucleotide chains of DNA and prevents reformation of hydrogen bonds between complimentary bases.
!!!!!! Phosphodiester bonds
Holds together adjacent nucleotides within a polynucleotide chain
!!!!!!Who found that DNA replicates semiconservatively?
Meselson and Stahl
!!!!! Who published the 3-dimensional structure of DNA?
Watson and crick
!!!!! Who found that DNA was the genetic material of T2 phage?
Hershey and chase
!!!!! Who found that the amount of adenine equals the amount of thymine and the amount of guanine equals the amount of cytosine, in any sample of DNA?
Chargoff
!!!!! Who found that DNA transferred avirulent bacteria into virulent bacteria?
Avery, Macleod, and McCarty
!!!!! Who found x-Ray diffraction indicated DNA was double stranded and uniform width?
Franklin
!!!!! Who found that rough streptococcus picked up something from dead smooth streptococcus that transformed them into the smooth type?
Griffith
Disaccharides:
Sucrose
Lactose
Maltose
Monosaccharide
Ribose
Hydrogen bonds along N-C-C backbone of polypeptide is important for what protein structure?
Secondary
What functional group isn’t attached to central carbon of an amino acid?
Phosphate group
Side chains (R groups) of polypeptide are important for what level of protein structure?
Tertiary
Protein denaturing involves:
Breaking hydrogen bonds along N-C-C backbone.
Functional groups on ends of polypeptide chain
Amino
Carboxyl
DNA complimentary hydrogen bonding occurs between:
One pyrimidine
One purine
!!!!!! What bond holds amino acids together in a polypeptide?
Peptide bond
What bond holds monosaccharides together in a polysaccharide?
Glycosidic linkage
What bond holds complimentary bases together in a DNA molecule?
Hydrogen bond
!!!!!! Triglycerides
Formed by bonding glycerol (3C molecule with hydroxyl group to each C) to 3 fatty acids
!!!!!! Primary structure level
Amino acid sequence from beginning to end.
-carry info for production of polypeptides with specific amino acid sequences
!!!! Secondary structure
Folding structure.
Causes protein to fold more compactly and in any shape.
!!!! Tertiary structure
Side chains of amino acids interact and polypeptide folds and refolds —-> 3D
!!!! Quaternary structure
2+ polypeptide chains that assembles with tertiary structure.
!!!! Factors that influence protein structure
H bonds
Ionic bonds