Exam 1- Enzymes Flashcards
Enzymes
Catalyze a reaction
Abzyme
Catalyze reactions and are made by attaching antibodies to the active site structure isolated from an enzyme (lab made)
Ribozymes
Made of RNA and can catalyze certain biochemical reactions
What does and does not change with enzymes?
Gibbs free energy the same
Activation energy lowered.
Enzyme specificity (4)
Steric hindrance
Arrangement of fixed charges
Hydrophobic surfaces
Residues capable of hydrogen bonding
Prosthetic groups
Small non-protein molecules associated with active sites of enzymes
Ex. Here, biotin or flavin
Holoenzyme vs apoenzyme
Holoenzyme: an enzyme with a prosthetic group
Apoenzyme: isolated protein part of a holoenzyme (without prosthetic group)
6 groups of enzymes
Oxidoreductases Transferases Hydrolases Leases Isomerases Ligases
Michaelis menten equation
V= Vmax x S/ Km + S
Michaelis Menten x and y axis. Where is V max and Km
X axis- S
Y axis- V
Top of hyperbole is the Vmax
X intercept at 1/2 Vmax= Km
Line weaver Burke. X and y axis and intercept
Y axis - 1/V
X axis- 1/S
Y intercept: 1/Vmax
X intercept: -1/Km
Eddie Hofstee x and y axis and intercept
Y axis- V
X axis- V/S
Y intercept- Vmax
X intercept- Vmax/Km
Competitive inhibitor
Increased Km, Same Vmax
Noncompetitive inhibitor
For other site
Vmax decreased, Km same
Uncompetitive inhibitor
Binds E-S complex
Vmax and Km decreased
Parallel lines on LB
