exam 1 Flashcards

1
Q

hematology

A

study of formed elements in blood
RBCs, WBCs, and platelets

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2
Q

chemistry

A

testing concentration of chemical constituents of bodily fluids and blood & blood collected in plasma separator/serum separator tubes

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3
Q

microbiology

A

planting specimens onto culture media

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4
Q

histology

A

study of tissue structure

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5
Q

example of accessioning

A

troubleshooting specimens and requisition errors

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6
Q

transfusion medicine

A

requires 2 EDTA tubes & providing blood components to patients

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7
Q

cytology

A

study of cells

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8
Q

coagulation

A

assess bleeding and clotting problems

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9
Q

example of hematology

A
  • bone marrow analysis
  • complete blood counts, ESR, white cell differential
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10
Q

example of microbiology

A
  • study of microorganisms
  • identification of pathogenetic bacteria
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11
Q

example of accessioning

A
  • distribute specimens to the appropriate testing department
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12
Q

example of transfusion medicine

A
  • called immunohematology
  • type and screen test
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13
Q

example of cytology

A
  • pap smear
  • cancer
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14
Q

example of coagulation

A
  • activated partial thromboplastin time
  • PT/INR
  • FIB tests
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15
Q

identifying critical results (pre, analytical or post)

A

post analytical

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16
Q

loading specimens onto analyzers (pre, analytical or post)

A

analytical

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17
Q

patient preparation for collection (pre, analytical, or post)

A

pre analytical

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18
Q

quality control procedure (pre, analytical, or post)

A

analytical

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19
Q

includes the majority of MLAs workload (pre, analytical or post)

A

pre analytical

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20
Q

specimen transport (pre, analytical or post)

A

pre analytical

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21
Q

disposal of old specimens and reagents (pre, analytical, or post)

A

post analytical

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22
Q

maintenance of automated instruments (pre, analytical, or post)

A

analytical

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23
Q

specimens collected properly (pre, analytical or post)

A

pre analytical

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24
Q

reporting and archiving results (pre, analytical, or post)

A

post analytical

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25
Q

tolerance is the same as *** and means:

A

accuracy
means: nearness to true value, or limits of error

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26
Q

precision is the same as ** and means:

A

reproducibility
means: repeatability

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27
Q

glassware calibrated at *** degrees celsius

A

20

28
Q

if a pipette is calibrated for both TC and TD, which marking will be the top one?

A

TD will be top mark

29
Q

what are volumetric pipettes used for?

A

accurate measurement of aqueous solutions

30
Q

why is the tip wiped before, not after, adjusting the meniscus to the calibration mark?

A

wiping draws some of the liquid out of pipette

31
Q

how will bubbles in a pipette tip affect the volume delivered

A

less volume will be delivered

32
Q

what should be done to an MLA pipette if the plunger doesn’t slide smoothly?

A

clean and lubricate the piston.

33
Q

what should be done to an MLA pipette if it doesn’t pick up the correct amount of sample

A

replace the seals

34
Q

what should be done to an MLA pipette if the nozzle is clogged

A

use a wire or blow air through

35
Q

how many stops does an eppendorf have

A

2 stops

36
Q

compare cylinders to volumetric flasks in terms of accuracy

A

volumetric flasks are more accurate

37
Q

when would a TC cylinder be used

A

to make less accurate solutions, to measure urine volumes

38
Q

when would a TD cylinder be used

A

to measure and deliver liquids into another container

39
Q

how can you determine if all detergent has been rinsed off glassware

A

use an indicator — changes colour at alkaline pH (from soap)

40
Q

the last rinsing must be done with ** water

A

distilled

41
Q

what type of filter used in a BSC

A

HEPA filters
high efficiency particulate air

42
Q

when does a safety cabinet require certification?

A

annually
when moved
when filter is replaced

43
Q

what is centrifuge used for

A

used to accelerate gravitational separation of substances

44
Q

what is the formula for calculating RCF

A

RCF = Krn^2

45
Q

can bleach be used to disinfect centrifuges? why

A

no, it will corrode metal

46
Q

what is the technique for using a serological pipette?

A

blow out the last drop at the tip

47
Q

identification of specimen integrity is part of post analytical workflows? T of F

A

false

48
Q

get to eyewash station within… and for how long

A

within 10 seconds for 15 mins

49
Q

safety showers have to be how long

A

atleast 15 mins

50
Q

biological spills

A

disinfectant soak for 30 mins

51
Q

tolerance and accuracy

A

refer to limits if error/nearness to true value

52
Q

precision and reproducibility

A

refer to ability to repeatedly produce the same value

53
Q

TC

A

to contain
glassware has been calibrated to HOLD the stated volume

54
Q

TD

A

to deliver
glassware is calibrated to DELIVER an accurate volume to another container

55
Q

refrigerator temp normally?

A

4 degrees celsius

56
Q

lab freezers usually at?

A

-70 degrees celsius and -30 degrees celsius

57
Q

BSC is used to?

A

provide workspace that prevents exposure to infectious aerosols

58
Q

k value is what

A

1.118x10-5

59
Q

the time required to separate mixers of varying densities depends on:

A

rotor speed
radius of rotor
effective path length traveled by the particles (depth of liquid in tube)

60
Q

less time is needed to centrifuge a specimen if:

A

speed (rpm) is increased
radius is increased
depth of liquid is decreased

61
Q

vortex

A

used to mix solutions in a quick, whirling motion

62
Q

rockers and mixers

A

used to gently mix many test tubes to provide homogenous samples

63
Q

reference electrode

A

produce a constant potential

64
Q

indicator electrode

A

contains glass membrane attracts hydrogen ions

65
Q

what is the most common test in microbiology

A

culture and sensitivity testing