Exam 1 Flashcards
microbe
a living organism that requires a microscope to be seen
supersized microbial cells
some species of protists grow to sizes large enough to see with the unaided eye
microbial communities
complex, multicellular assemblages i.e. mushrooms, kelps
virus
a noncellular particle containing genetic material that takes over the metabolism of a cells to generate more virus particles
Florence Nightengale
- founder of professional nursing and the science of medical statistics
- used methods by the French to prove the high mortality rate due to disease among British soldiers
Robert Hooke
- built a compound microscope
- saw nematodes
- never saw single cell bacteria
Leeuwenhoek
- first to observe single-cell microbes (really)
- saw lice/fleas and single cell protists and algae
spontaneous generation
the theory that under current earth conditions life can arise spontaneously from nonliving matter
Spallanzani
- showed a sealed flask of meat brother sterilized by boiling failed to grow microbes
- also demonstrated cell fission
Pasteur
- addressed spontaneous generation
- revealed biochemical basis of microbial growth
- found metabolic preference for one mirror image of a molecule over another
fermentaion
the production of ATP via substrate-level phosphorylation, using organic compounds as both electron donors and acceptors
Pasteur discovered _____ is caused by _____ in the swan neck experiemtn
fermentation, yeast
Tyndall
- performed similar experiment to Pasteur but found opposite result
- hay infused broth gave rise to endospores
- lead to autoclave
endospore
heat resistant form of bacteria
germ theory of disease
the theory that many disease are caused by microbes
chain of infection
the serial passage of a pathogenic organism from and infected individual to a healthy one
pure culture
a culture containing only a single strain of species of microorganism
colony
a visible cluster of microbes on a plate, all derived from a single founding microbe
Koch’s postulates (4)
- the microbe is found in all cases of the disease but is absent from healthy individuals
- the microbe is isolated from the diseased host and grown in pure culture
- when the microbe is introduced into a healthy individual, the host shows the same disease
- he same strain shows the same characteristics as before
antiseptic
a chemical that kills microbes
Lister
- started using antiseptic agents to treat wounds and surgical instruments
- developed fully antiseptic environment
- antiseptics, if ingested, killed patients so it lead to the search for antibiotics
lithotroph
organisms that feed solely on inorganic minerals, aka demolithotrophs
Winogradsky
- among the first to study microbes in natural habitats
- discovered lithotrophs
enrichment culture
the use of selective growth media to only allow certain microbes to grow