Eukaryotes Flashcards
Eukaryotes have
Many subcellular components (organoids) bound by an internal plasma membrane
Primary feature of Eukaryotes is that
Their primary nuclear material (DNA) is bound within a membrane (Nuclear Envelope)
Nuclear Envelope
Membrane that contains DNA
6 Main differences of Eukaryotic cells compared to Prokaryotic cells
-greater size
-nucleus is present with linear chromosomes
-histones are present
-membrane bound organelles in cytoplasm
-flagella complexity
-cell wall structure differs (no capsule present)
Multicellular Eukaryotes include
-plants
-fungi
-animals
Histones
Proteins that bind DNA for regulation
Which Eukaryotes have a cell wall?
-plants
-fungi (composed of chitin)
Epithelial Cell Junctions
Adhesion structures and molecules within external membrane that keep everything in place (animals only)
5 Features of a Eukaryotic Cell
-membrane bound organelles
-nucleus
-protein histone for regulation of DNA organization
-complex genetic expression
-cytoskeleton containing Actin and Tubulin
Eukaryotic Cells contain 5 main types of organelles:
-nucleus
-cytoplasm
-cytoskeleton
-mitochondria and plastids
-chloroplasts (plants)
Nucleus (4)
-control centre of cell
-contains genetic material (DNA) organized into Chromosomes
-site of synthesis of RNA (transcription)
-contains Histones (regulatory proteins that control how cells transcribe DNA)
Nucleus is surrounded by the
Nuclear Envelope/Membrane
Nucleolus
Part of the nucleus that makes ribosomes
What is the Cytoplasm? What are some components found in it?
-rest of the cell
Components include:
-endomembrane system
-ribosomes
-cytoskeleton
-mitochondria and plasmids
-plasma membrane
-other inclusions
Egress
Molecules within nucleus, synthesized in cytoplasm, and secreted/released from cell
Ingress
Molecules/structures that are detected at the surface of the cell and taken in via membrane systems
Three functions of the Cytoskeleton + what it contains (2)
-structure/shape
-movement of cell and within cell
-control of cell division
-contains Actin and Tubulin
Actin and Tubulin are found
In the Cytoskeleton of Eukaryotic cells
Four functions of Mitochondria
-powerhouse of cell
-generates ATP (energy)
-makes Haem and specialized proteins
-cell signalling, control of cell cycle
Mitochondria have their own
DNA that replicates
Chloroplasts
-found only in plants
-have their own DNA
-convert light energy into chemical energy
-one of many plastids
Plant cells differ from animal cells because they have
-cell wall and plasmodesmata
-chloroplasts
-central vacuole
Animal cells differ from plant cells because they contain
-centrioles
-lysosomes
Phylogenetic Tree is
A graphic representation of hypothetical origin of species or Taxa (groups of species) over time
Branch Point in Phylogenetic Tree
Shows the last common ancestor of taxa
Branches in Phylogenetic Tree represent
Divergence of species
Three domains of all life forms include
-Bacteria (Prokaryota)
-Archaea (Prokaryota)
-Eukaryotes (Eukaryota)
Serial Endosymbiosis Theory
-theory on how Eukaryotes rose
-cell membrane infolded in a bacterial cell —> eventually, the infolded membrane surround nuclear material (DNA) —> this advanced cell engulfed other bacterial cells that eventually became organelles
Theory of the origins of flagella, plastids, and mitochondria
Endosymbiotic Origin
3 pieces of Evidence that Endosymbioses is the origin of Eukaryote organelles:
-occurs today
-mitochondria and plastids have their own genomes and similar structures
-genetic sequence of DNA in mitochondria is similar to that of some prokaryotes
6 Similarities between mitochondria and bacteria
-size and morphology
-bound by double membrane like gram-negative bacteria
-similar enzymes and inner membrane transport systems
-binary fission
-circular DNA
-own protein synthesis machinery including rRNA and tRNA
Example of current Endosymbioses
Mixotricha paradoxa (termites) —> functions as a symbiotic assemblage of five different species living to form one living cell
Endosymbiont
An organism that lives within another organism
Autotrophy
Ability to be self-sustained by producing foods from inorganic compounds
Example: Algae
Heterotrophy
Food is sourced from organic compounds
Example: animals
Single Celled Eukaryotes (SCE)
Unicellular organisms
Most eukaryotes are
Single celled
Eukaryota can be divided into three kingdoms
-Animals
-Plants
-Fungi
SCE clades/groups are classified based on these 5 features
-molecular sequence
-cell ultrastructure
-presence/absence of locomotory structures
-presence/absence of mitochondria and plastids
-structure of membrane
5 super groups of SCE
-Flagellates
-Alveolata (Apicomplexans and CIliates)
-Amoebozoans
-Opisthokonts
-Plant-like Organisms (red and green algae)
Flagellates are also called
Excavata
Flagellate sub groups include
-Diplomondida (ex. Giardia)
-Euglenozoa (ex. Trypanosoma)
Flagellates / Excavata have 3 key features
-anterior (front end) falgella
-usually 2+ flagella per cell
-highly modified mitochondria
How do Flagella and Cilia differ?
Flagellum: (Latin for whip)
-long, threadlike, on surface of cell
-wave-like and slow beat
Cilium: (Latin for eyelash)
-short, hairlike, on surface of cell
-abundant
-fast and rotating beat
Diplomonadida
-sub group of flagellates
-lack mitochondria (possess “Mitosomes”)
-lack plastids
-mostly anaerobic
-mostly symbiotic
-many are parasitic
Example: Giardia
Mitosomes
Mitochondria-like organelle but not used for ATP generation
Locomotory appendages are called
Flagella
Symbiotic
Live within other organisms
Parasites
Cause harm to the organism they live in
Anaerobic
Adapted to life in low oxygen
Giardia
-part of Diplomonadida, Flagellates
-intestinal parasite containing resistant cysts that contaminate water supply
Kinetoplastids
-Parasitic Euglenozoids (Flagellates)
-mitochondria has a large circular DNA body
-heterotrophs
-free living and can be parasitic
Example: Trypanosoma
Trypanosoma
-type of Kinetoplastid (Euglenozoid, Flagellate)
-some species live in circulatory system of vertebrates
-responsible for disease (Nagana in animals, Sleeping Sickness in humans, Chagas Disease)
Diplomonadida
Sub group of Flagellates
Euglenozoa
Sub group of flagellates
Diplomonadida lack
Mitochondria
Most species of Diplomonadida are
Anaerobic
Giardia is an ____ parasite
Intestinal
Circle of DNA inside mitochondrion is called
Kinetoplast
Kinetoplastids divide via
Binary fission
Three examples of Kinetoplastids
-African Trypanosomes (sleeping sickness/nagana)
-New World Trypanosome (Chagas Disease)
-Leishmaniasis