Ethnopharmacology 2 Flashcards
Use of POLAR or NON POLAR SOLVENTS to RECOVER desired components
Extraction
Capable of getting the compound
Extraction
Simple extraction includes
Stirring
Percolation
Complex extraction includes
Column
Pressurized
DIFFERENTIATES the bioactives from the non bioactives
Isolation
Isolation involves
Separation method (Chromatography)
Determine the ATOMS and FUNCTIONAL GROUPS as well as unveil STEREOCHEMISTRY
Identification
Identification includes
Structure elucidation
Carbon skeleton
Detailed picture with electron-density maps
To identify the COMPOUND and FUNCTIONAL GROUPS to readily manipulate or optimize its effects
Structure elucidation
Example to show the carbon skeleton
NMR
Example of detailed picture with electron-density maps
X-ray crystallography
Goal of identification
Identify specific compounds and replicate it
Use of STABILITY TECHNIQUES to avoid alterations in molecules
Standardization
It should be REPEATED to make sure it is correct
Standardization
Standardization is for
Storage conditions
- how factors in the environment can affect your extract