ETC - online questions Flashcards
1 NADH = How many ATP
2.5 ATP
1 FADH2 = How many ATP
1.5 ATP
How many H+ ions does it take to make 1 ATP
4 H+
1 NADH pumps how many H+ across
10
1 FADH2 pumps how many H + across
6
TCA occurs where
Matrix of mitochondria
OP Occurs where
Inner membrane of mitochondria
At the last step of OP each NADH or FADH2 produces what?
One water molecule
1 Glucose molecule = how many ATP
32 ATP (experimental yield)
Explain how NADH goes through the electron transport chain
Complex I > UQ > Complex 3 > Cytochrome C > Complex 4
Name each complex (4 of them) in the electron transport chain and how many hydrogen’s are pumped across
Complex I - 4 H+
Which complex is water created by
Complex IV
What kind of gradient is created by the electron transport chain
A chemical and electrical gradient
ATP Synthase is made up of what
F0 and F1
F0 is
The porin part or the part that lets the H+ ions go through it
F1 is
The active site for the ADP > ATP reaction
What are the 3 active sites in ATP Synthase in order
L > T > O
Which is more negative ADP or ATP and why is this important?
ATP is more negative ; allows it to get out of the mitochondria due to the electrical gradients
How do NADH from glycolysis get into the mitochondria?
2 Different Shuttle Mechanisms
The Glycerophosphate Shuttle does what
NADH > FADH2
What enzyme is used?
Glycerophosphate Dehydrogenase
Malate Asparate Shuttle does what?
NADH > NADH
Explain what happens
Oxaloacetate > Aspartate > goes and gets electrons off NADH > goes back into mitochandria > Oxaloacetate
Retenone is an insecticide that does what
Inhibits complex I of the ETC
Demerol or Meperidine does what
Inhibits complex I
Amytal does what
Inhibits complex I
Antimycin does what
Inhibits complex III
Myxothiazol
Inhibits complex III
Cyanide and Carbon Monoxide
Inhibit complex IV
Oligomycin is a
Macrolide
What does a macrolide (class drug) do?
Binds to the F0 or porin part of ATP Synthase and blocks proton flow
Arsenic does what
Inhibits pyruvate dehydrogenase therefore we cannot enter the TCA cycle
