Etc Flashcards
NADH carries
2 e and 1 H+
NADH reversible?
Yes
FADH (FMNH) carries
1 e and 1 H+
FADH2 (FADH2) carries
2 e and 2 H+
FAD/FMN reversible?
tightly bound to proteins
FAD and FMN reduction potential
vary significantly with protein microenvironment
*QH carries
1 e and 1 H+
QH2 carries
2 e and 2 H+
coenzyme Q reversible?
yes
hemes carry
1 e-
heme reversible?
tightly bound or covalently bonded to proteins
cytochrome
protein with bound heme
Fe-S clusters carry
each Fe in an Fe-S cluster can carry 1 e
Fe-S clusters reversible?
covalently bonded to proteins by cysteine side chain sulfur ligands
Cu centers carry
1 e
Cu centers reversible?
covalently bonded to proteins by cysteine side chain sulfur ligands
intermembrane space charge
postitive
matrix charge
negative
what does the matrix contain
PDH, enzymes of the citric acid cycle, FA degradation pathways
complex 1 input substrate
NADH
complex 1 output e-carrier
coenzyme Q
complex 1 number of H+ pumped to P side
4
complex 1 number of H+ pumped to QH2
2
complex 2 input substrate
succinate
complex 2 output substrate
coenzyme Q
complex 2 number of H+ pumped
0
complex 3 input substrate
coenzyme Q
complex 3 output substrate
Cytochrome C
complex 3 number of H+ pumped to P side
4
complex 4 input substrate
cytochrome c
complex 4 output e carrier
complex 4 number of H+ pumped to P side
2
order of ETC cofactors
- NAD
- FAD/FMN
- Coenzyme Q
- Hemes
- Fe-S clusters
- Cu clusters
ways that NADH can enter the ETC
Malate/Asp
Lactate shuttle
TCA
ways that FADH2 can enter the ETC
TCA
Glycerol phosphate shuttle
which verision of coenzyme Q is fullly oxidized
ubiquinone
which verision of coenzyme Q is fully reduced
uniquinol
which verision of coenzyme Q is partly reduced
semiquinone
P/O ratio
ATP produced per O2 reduced
% effiency
eoutput/einput or delta g transport/delta g hydrolysis
delta G of moving a proton across the membrane
+ 18.9 kJ/mol
how many H+ pumped per NADH
10