Equipment in the Component Manufacturing Laboratory

Question 1

very crucial (speed and duration), can spin 6 to 12 units

Answer 1

Centrifuge

Question 2

typically either large floor units or large table top units that can spin a maximum of 6 to 12 units of whole blood at once.

Answer 2

Centrifuge

Question 3

Purpose of centrifuge:

Answer 3

Separate red blood cells from plasma and other components

Question 4

Centrifugation depends on the following conditions:

Answer 4

• Length/duration
• Speed

Question 5

Recommended Centrifuge Conditions

Answer 5

AABB

Question 6

• Red blood cells + plasma
• Soft spin

Answer 6

5000 x g = 5 minutes

Question 7

Red blood cells + PRP

Answer 7

2000 x g = 3 minutes

Question 8

• Harvesting platelets or cryoprecipitate from plasma
• Hard spin

Answer 8

5000 x g = 7 minutes

Question 9

platelets that are not entirely removed from the plasma

Answer 9

PRP

Question 10

Methods of Centrifugation

Answer 10

1. Intermittent Flow Centrifugation (IFC)
2. Continuous Flow Centrifugation (CFC)

Question 11

• Blood is processed in BATCHES or CYCLES, hence the term intermittent
• Whole blood is drawn from an individual with the ASSISTANCE of a PUMP

Answer 11

Intermittent Flow Centrifugation (IFC)

Question 12

In IFC, To keep the blood from clotting, an ___ is mixed with the ____ as it is pumped into a centrifuge _____ through the inlet port

Answer 12

anticoagulant
blood
bowl or chamber

Question 13

• the processes of blood withdrawal, processing, and reinfusion are performed SIMULTANEOUSLY in an ongoing manner
• COMPLETE a cycle before beginning the next one

Answer 13

Continuous Flow Centrifugation (CFC)

Question 14

In CFC, blood is DRAWN and RETURNED continuously during a procedure, ____ venipuncture sites are necessary

Answer 14

two

Question 15

Plasma products that are REMOVED/SEPARATED from the red blood cell

Answer 15

Plasma Expressors

Question 16

Mechanical devices that apply PRESSURE to the blood bag, which allows blood components to FLOW from ONE BAG TO ANOTHER by way of the INTEGRATED tubing system

Answer 16

Plasma Expressors

Question 17

____ help prepare a component for another therapy indications

Answer 17

Expressors

Question 18

The tubing that connects blood bags is usually made of polyvinyl chloride (PVC)

Answer 18

Tubing Sealers

Question 19

reliable component of hospital equipment, resistant from damages such as high temp etc

Answer 19

polyvinyl chloride or PVC

Question 20

Allow TWO SEPARATE blood bags to be CONNECTED via their PVC tubing WITHOUT breaching the integrity of either container.

Answer 20

Sterile Connection Devices

Question 21

In Sterile Connection Devices, each piece of tubing is placed in a ____ and ____

Answer 21

channel
clamped

Question 22

Blood component ____ should be carefully SELECTED and VALIDATED to ensure that they are capable of MAINTANING the FDA-regulated storage temperatures

Answer 22

storage devices

Question 23

In Storage devices, Temperatures must be ____ monitored, recorded at least every ____, and the device should ____ the user if an unacceptable temperature condition occurs.

Answer 23

continuously
4 hours
alert

Question 24

Usually, hospitals are using TRIPLE bags depending on the component that will be produced. In earlier times, the BOTTLE is used for the storage of blood

Answer 24

Quadruple bag

Question 25

• _____ – where the PRIMARY component is stored (whole blood)
• _____ - CONNECTED through the TUBING for storage of separated components

Answer 25

Mother Bag
Satellite Bags

Question 26

Preparation Protocol (WCCE)

Answer 26

1. Weighing
2. Counter balancing
3. Centrifuge
4. Expression

Question 27

In Preparation protocol, ____ is to sure that the blood bag is SOMEWHAT the SAME with its balancer

Answer 27

Weighing

Question 28

In Preparation protocol, ____ is to COUNTER BALANCE the blood bag

Answer 28

Counter balancing