Enzymology

Question 1

What Enzyme Class is Ligase?

Answer 1

Enzyme Class 6 (EC6)

Question 2

Give an example of Isomerase

Answer 2

Phosphoglucoisomerase

Question 3

Name an example of Hydrolase

Answer 3

Chymotrypsin

Question 4

What Enzyme Class is Isomerase?

Answer 4

Enzyme Class 5 (EC5)

Question 5

Give an example of Oxioreductase

Answer 5

G3P Dehydrogenase–Glyceraldehyde 3-P reacted with NAD+ to get 1,3- Biphosphoglycerate

Question 6

If water is present with one substrate, but two products are formed, what enzyme class would most likely be present?

Answer 6

EC3-Hydrolase

Question 7

What is the main function of Lyase

Answer 7

Bond cleavage by elimination

Question 8

Name the 6 Classes of Enzymes

Answer 8

1. Oxioreductase
2. Transferase
3. Hydrolase
4. Lyase
5. Isomerase
6. Ligase

Question 9

What is the main function of Isomerase

Answer 9

Intermolecular rearragement

Question 10

If substrate and product have the same molecular formula but different structure, what enzyme class is most likely present?

Answer 10

EC5 - Isomerase

Question 11

What is NTP

Answer 11

Nucleic Triphosphate (ATP, CTP, TTP, GTP)

Question 12

What is the main function of Oxioreductase

Answer 12

Transfer of Electrons

Question 13

What is the main function of Ligase

Answer 13

NTP-Dependent bond formation

Question 14

What is the main function of Transferase

Answer 14

Transfer of functional groups

Question 15

What Enzyme Class is Transferase?

Answer 15

Enzyme Class 2 (EC2)

Question 16

What Enzyme Class is Oxioreductase?

Answer 16

Enzyme Class 1 (EC1)

Question 17

What Enzyme Class is Hydrolase?

Answer 17

Enzyme Class 3 (EC3)

Question 18

If there is one substrate and two products but no net water, what enzyme class is most likely present?

Answer 18

EC4 - Lyase

Question 19

What should I recognize as a Hydrolase reaction?

Answer 19

Water with ONE substrate, TWO products

Question 20

If NAD+ is present, what enzyme class is most likely present?

Answer 20

EC1 - Oxioreductase

Question 21

Give an example of Transferase

Answer 21

Hexokinase and ATP - one of the phosphate groups gets added onto the substrate

Question 22

What should I recognize for a transferase reaction?

Answer 22

Same number of products as there are substrates. A small amount of one substrate ends up on the other.

Question 23

What is the main function of Hydrolase

Answer 23

Single bond cleavage by the leaving of water

Question 24

What Enzyme Class is Lyase?

Answer 24

Enzyme Class 4 (EC4)

Question 25

What should I recognize as a Isomerase recation?

Answer 25

Typically one substrate and product–same number of substrates and products. Substrate and product have the same molecular formula.

Question 26

What should I recognize as a Ligase reaction?

Answer 26

An NTP is present but not incorporated into the products (phosphate group is not added onto the product). Some substrate is added onto another substrate.

Question 27

Give an example of Lyase

Answer 27

Fructose biphosphate aldolase

Question 28

If there are the same number of products as there are substrates, what enzyme class is most likely present?

Answer 28

EC2 - Transferase

Question 29

What should I recognize as a Lyase reaction?

Answer 29

No water present. One substrate and two products.

Question 30

What should you recognize for an oxioreductase reaction?

Answer 30

1. NAD+/NADH; FAD/FAD+; Q/QH2
2. Product has more Oxygens than substrate