Enzymes and Temperature - Practical Skills

Question 1

Write the word equation for the action of amylase

Answer 1

Starch > Maltose

Question 2

How is the rate off amylase activity measured?

Answer 2

Testing a drop of the enzyme substrate mixture at fixed time intervals with iodine solution to find the time taken for the iodine solution to remain brown

Calculate rate = 1/time taken

Question 3

What is indicated by the iodine solution remaining brown when tested?

Answer 3

All starch as been hydrolysed by amylase

Question 4

Why should the solutions be left in a water bath for a few minutes before being mixed together?

Answer 4

To allow the temperature to equilibrate

Question 5

How can the rate be more accurately determined?

Answer 5

By testing the presence of starch in the mixture at smaller time intervals

Question 6

How can the estimate of the optimum temperature be improved?

Answer 6

Repeat the practical at smaller intervals of temperature around the estimated optimum temperature

Question 7

State the safety precautions involved in this practical

Answer 7

• Safety goggles should be worn
• Be carful with water and electrical equipment