Enzymes and Factors that Affect Enzyme Activity

Question 1

Provide energy for the maintenance of life-support systems of the organism

Answer 1

metabolic processes

Question 2

metabolic process consists of degrading chemical compounds of ____ to products with ___

Answer 2

relatively high potential energy
relatively low potential energy

Question 3

example of degradation is glucose to

Answer 3

CO2 and H2O

Question 4

the energy evolved is

Answer 4

collected
stored
utilized by cell for growth
reproduction
synthesis
repair of cellular materials

Question 5

this degradation process is made possible by the presence of catalytic substances called

Answer 5

enzymes

Question 6

they alter the energy of activation

Answer 6

catalysts

Question 7

increasing the energy of activation

Answer 7

decreases the rate of reaction (negative catalysis)

Question 8

decreasing the energy of activation

Answer 8

increases the rate of reaction (positive catalysis)

Question 9

are complex organic substances produced by an organism to alter the rate of biochemical reactions that take place in the cell.

Answer 9

enzymes

Question 10

true or false: enzymes show high degree of specificity

Answer 10

true. only one kind of reaction for one kind of substrate

Question 11

the hydrolysis of sucrose is catalyzed by

Answer 11

invertase (acts only in sucrose, not other disaccharides)

Question 12

true/false. HCl, in vitro, will hydrolyze a number of disaccharides and even polysaccharides

Answer 12

true. no specificity since not enzyme

Question 13

an enzyme derived from potatoes and which detoxifies peroxides produced in the body from the metabolism of amino acids

Answer 13

catalase

Question 14

factors that denature proteins

Answer 14

1. extremes of pH and temperature
2. metal and non-metal ions
3. presence of alkaloidal reagents
4. enzyme and substrate concentration

Question 15

Procedure I. A Preparation of enzyme materials/reagents/process

Answer 15

1. Potato peel
2. add 50 mL water (stand for 10 mins)
3. strain through cheesecloth
4. get 5 mL of the filtrate
5. mix with 5 mL of 6% H2O2

Question 16

Preparation of enzyme. what gas was indicated in Preparation of enzyme

Answer 16

oxygen (product of catalase breaking down hydrogen peroxide to water and

Question 17

1. B Protein nature of enzyme materials/procedure

Answer 17

1. 5 mL of catalase extract
2. mix with 5 mL 3 M NaOH
3. add 5 drops CuSO4

Question 18

1. B protein nature of enzyme observation

Answer 18

mixed w/ NaOH - light yellow
5 drops CuSO4 - clear blue precipitate

Question 19

T1 Effects of Ph reagents

Answer 19

10 ml 1% starch solution + 0.1 M NaCl + 1 mL 0.05 N HCL + 2 mL saliva

Question 20

T2 Effects of Ph reagents

Answer 20

10 ml 1% starch solution + 0.1 M NaCl + 1 mL distilled water + 2 mL saliva

Question 21

T3 Effects of Ph reagents

Answer 21

10 ml 1% starch solution + 0.1 M NaCl + 1 mL 0.05 N NaOH + 2 mL saliva

Question 22

what is the optimum pH of salivary amylase

Answer 22

6-7, highest activity at approx. 6.8

Question 23

pH. water

Answer 23

hydrolyzes starch fastest

Question 24

ph. NaOH

Answer 24

hydrolyzes slower due to alkalinity

Question 25

ph. HCl

Answer 25

barely hydrolyzes due to its acidic nature

Question 26

equation for the reaction catalyzed by catalase

Answer 26

2H2O2 (aq) —–> 2H2O (l) + O2 (g)

Question 27

how does enzyme alter the rate of chemical reaction

Answer 27

by reducing the activation energy, increasing chemical reaction

Question 28

optimum temp of salivary amylase

Answer 28

32 degree C and 37 degree C, peak at 37

Question 29

at lower temp enzyme gets

Answer 29

deactivated

Question 30

at higher temp enzymes gets

Answer 30

denatured