Enzymes Flashcards
1
Q
What are the 2 sites on an enzyme?
A
Substrate binding site
Catalytic site
2
Q
What conditions are needed to create an enzyme-substrate complex?
A
- close proximity of enzyme and substrate
- orientation of the 2 substrates
- strain/distortion
- acid/base catalyst
3
Q
What is 1 katal?
A
1 katal of enzyme activity produces 1 mole of product/second
OR utilizes 1 mole of substrate/second
4
Q
What is 1 unit in terms of enzyme activity?`
A
1 unit of enzyme activity produces 1 micromole of product/minute
OR utilizes 1 micromole of substrate/minute
5
Q
What is turnover number (Kcat)?
A
The number of substrate molecules that can be converted to producgt by 1 enzyme in 1 second
