Enzymes

Question 1

Collision theory

Answer 1

-enzyme & substrate must collide.
- they must collide in the correct orientation.
- they must collide with atleast the minimum every to initiate the reaction (activation energy)
- changing temp concentrations (of substrate or enzyme), changing surface area of substrate can all impact the frequency of collisions & the rate of reaction.

Question 2

Lock & key model

Answer 2

• The shape of the active site is unique, complementary & specific to the substrate.
• Once the enzyme binds the substrate this is called the “enzyme -substrate complex”
• enzyme then catalyses the reaction forming new products.

Question 3

Factors that affect rate of reaction

Answer 3

• Higher temp (increase frequency of collisions)
• higher concentration
• greater SA

Question 4

Induced fit model

Answer 4

• Substrate binds to shape of active site to be a better fit.
• enzyme binds= enzyme-substrate complex

Question 5

Name when active site loses shape & prevents binding of substrate.

Answer 5

Denature

E.g = temp too high compared to optimum can cause denaturing

Question 6

Competitive inhibitor?

Answer 6

-bindto active site & compete with substrate for binding

• reversible

Question 7

Non - competitive inhibitor?

Answer 7

• Bind to another site on the enzyme = not active site.
• irreversible