Enzymes Flashcards
Enzymes may recognize and catalyze:
⊚ A single substrate
⊚ A group of similar substrates
⊚ A particular type of bond
Acted upon by the enzyme and specific
Substrate
Acted upon by the enzyme and specific but different form with the same action
Isoenzyme
Non-protein molecule of a conjugated enzyme
Cofactor
Activator/Metal ions
Inorganic cofactor
Organic cofactor
Coenzyme
Polypeptide portion of a conjugated enzyme and inactive enzyme
Apoenzyme
Apoenzyme + Cofactor
Holoenzyme
⊚ Also known as zymogen
⊚ Inactive form or precursor of enzyme
⊚ It is then converted, usually by proteolysis,
to the active form when it has reached the site of its activity
Proenyzme
Enzyme Commission Nomenclature (E.C.)
⊚ 1st digit – class
⊚ 2nd digit – subclass
⊚ 3rd and 4th digit – serial number
Oxidation and reduction
Oxidoreductases
Transfer of functional groups other than
hydrogen from one substrate to another
Transferases
transfer of amino group
Transaminases
transfer of phosphate group from
ATP to ADP + phosphorylated product
Kinases
• is an enzyme that catalyzes a hydrolysis
reaction in which the addition of a water molecule to a bond causes the bond to break
• Central to the process of digestion
Hydrolases
breaking of glycosidic bonds in oligosaccharides and polysaccharides
Carbohydrases
breaking of peptide linkages in proteins
Proteases
breaking of ester linkages in triglycerides
Lipases
• is an enzyme that catalyzes the addition
of a group to a double bond or the removal of a group to form a double bond in a manner that does not involve hydrolysis or oxidation
• any member of a class of enzymes that catalyze the addition or removal of the elements of water (hydrogen, oxygen), ammonia (nitrogen, hydrogen), or carbon dioxide (carbon, oxygen) at double bonds
Lyases
removal of the components of water from a double bond
Dehydratase
addition of water to a double bond
Hydratase
catalyze the removal of CO2 from a substrate
Decarboxylases
catalyze the removal of NH3 from a substrate
Deaminases
catalyze the removal of H2O from a substrate
Dehydratases