Enzymes

Question 1

Temperature

Answer 1

Optimal temperature -> maximum enzyme substrate collisions, increased rate of reaction.
Too high -> disulfide bridges and hydrogen bonds will break making the enzyme lose its shape and substrate and active site don’t bond and enzyme is denatured. Reaction won’t happen.
Too low -> molecules move slowly, less enzyme substrate collisions therefore lower rate of reaction.

Question 2

pH

Answer 2

Optimal pH -> shape of active site is conserved
Extreme change -> disrupts hydrogen bonds and changes shape of active site, rate of reaction slowed or won’t happen.

Question 3

Inhibitors

Answer 3

Bind to active site so substrate cannot fit or they bind to the outside of an enzyme in a way that the active site changes. Substrate can no longer bind, reaction doesn’t happen.

Question 4

Enzyme Concentration

Answer 4

Higher enzyme conc leads to an increased ROR as more substrate enzyme reactions are happening. Rate of reaction will level out once all substrates are bound to an enzyme.

Question 5

Substrate Concentration

Answer 5

Higher substrate concentration leads to an increased ROR as more substrate enzyme reactions are happening. Rate of reaction will level out once all enzymes are bound to a substrate.

Question 6

Co-factors and Co-enzymes

Answer 6

Co enzymes and organic (include carbon) and co factors are inorganic (don’t include carbon).
Some enzymes need a co-factor/co-enzyme to complete its active site.