Enzymes

Question 1

made in what organelle?

Answer 1

ribosomes

Question 2

Why do pH and temp effect enzyme activity

Answer 2

denatured, the shape of active site is altered

Question 3

How enzymes work

Answer 3

Shape of active site changes slightly to fit substrate of corresponding shape (induced fit model), enzyme substrate complex, products released, active site is unaltered

Question 4

immobilized enzyme

Answer 4

trapped in an inert material

Question 5

advantage of immobilized enzymes

Answer 5

reusable

Question 6

What is meant by ‘specificity’ in relation to enzymes

Answer 6

only acts on one substrate

Question 7

Why, in protein synthesis, is protein folded into specific shape

Answer 7

To produce functional protein

Question 8

Is production of enzymes catabolic or anabolic? Why?

Answer 8

Anabolic. Larger protein molecule formed from smaller amino acids

Question 9

Temperature expt: enzyme, substrate, product

Answer 9

Catalase, H202, 02

Question 10

Temp at which enzyme is denatured

Answer 10

60+ degrees C for at least 5 minutes

Question 11

How do you keep pH constant in experiment?

Answer 11

pH buffer