Enzymes Flashcards

1
Q

All enzymes are

A

catalysts

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2
Q

Enzymes do what?

A

increase rate of reaction by lowering activation energy

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3
Q

True or False enzymes are NOT consumed in reaction

A

True

Enzymes NOT consumed in reaction

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4
Q

Molecule which enzymes act upon

A

substrate

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5
Q

Enzymes act on

A

substrates

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6
Q

Measure of affinity that an enzyme has towards the substrate

A

Km

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7
Q

decrease in KM will increase AFFINITY

A

inverse relationship

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8
Q

Max velocity of an enzyme

A

VMAX

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9
Q

Km = of VMAX

A

Km = ½ of max

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10
Q

3 factors affecting reaction velocity

A
  1. substrate concentration
  2. Temperature
  3. pH
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11
Q

Maximal velocity of an enzyme catalyzed reaction

A

VMAX

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12
Q

Amount of substrate required to reach ½ of VMAX

A

Km

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13
Q

Increase in KM

No Change in VMAX

^ -

A

Competitive Inhibitors

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14
Q

No change in Km

Decrease in VMAX

-V

A

Noncompetitive Inhibitor

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15
Q

Competitive inhibitors bind to

A

substrate binding site of an enzyme

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16
Q

Competitive Inhibitors will

A
  1. increase Km and lower affinity
  2. no change max
  3. add enough substrate to dilute out inhibitors
17
Q

Noncompetitive inhibitors will bind to

A

site other than substrate binding site

18
Q

Noncompetitive inhibitors will

A
  1. no change in Km
    1. decrease VMAX will take longer to reach VMAX
19
Q

measure of amount of energy available to determine if reaction is feasible

A

Gibbs Free Energy

20
Q

Delta G = 0

A

equilibrium

21
Q

Delta G = +

A

endothermic or endergonic

nonspontaneous

unfavorable

22
Q

Delta G = -

A

exothermic exoergonic

spontaneous and favorable