Enzymes Flashcards
All enzymes are
catalysts
Enzymes do what?
increase rate of reaction by lowering activation energy
True or False enzymes are NOT consumed in reaction
True
Enzymes NOT consumed in reaction
Molecule which enzymes act upon
substrate
Enzymes act on
substrates
Measure of affinity that an enzyme has towards the substrate
Km
decrease in KM will increase AFFINITY
inverse relationship
Max velocity of an enzyme
VMAX
Km = of VMAX
Km = ½ of max
3 factors affecting reaction velocity
- substrate concentration
- Temperature
- pH
Maximal velocity of an enzyme catalyzed reaction
VMAX
Amount of substrate required to reach ½ of VMAX
Km
Increase in KM
No Change in VMAX
^ -
Competitive Inhibitors
No change in Km
Decrease in VMAX
-V
Noncompetitive Inhibitor
Competitive inhibitors bind to
substrate binding site of an enzyme