enzyme test Flashcards
most enzymes need a certain:
temperature and pH range
if enzymes are exposed to conditions outside their designated temp and pH range,
they could denature
decomposition reaction
when a large molecule is broken down into 2 smaller molecules
synthesis reaction
when 2 or more smaller molecules are combined to form a larger molecule
enzyme – used again
can be
3 steps to how enzymes work
- substrate(s) bind(s) to enzyme’s active site
- Enzyme substrate complex (reaction occurs)
- new products are released
active site
region where the reaction takes place
specific shape
substrates
reactants
specific shape
bind to active site
enzyme names usually end in
“ase”
enzymes are named after:
the substrate they act upon
enzymes act as
catalysts
catalysts
lower the activation energy of a chemical reaction
enzymes
special proteins
critical to life
most enzymes are what type of macromolecule
proteins
3 examples of enzymes ending with ase
RNA polymerase
dehydrogenase
catalyse
to denature an enzyme means the enzyme becomes – and can no longer work properly
unfolded
macromolecules
carbs
lipids
nucleic acids
proteins
monomer of carbohydrates
monosaccarides
monomer of lipids
glycerol and fatty acids
monomer of nucleic acids
nucleotides
monomer of proteins
amino acids
what effects enzymes
temp
pH
substrate concentration
salinity
testing for carbs presence in food
iodine solution changes from yellow/brown to purple/black in presence of starch (complex sugar)
testing for lipid presence in food
lipids leave translucent spots on uncoated brown paper bags
testing for protein presence in food
Buriet solution changes from blue to purple in the presence of proteins
