animal biotech Flashcards
Animal biotech
application of scientific engineering to the processing of materials by animals to provide goods and services
functional foods
have a positive effect on health beyond basic nutrition
artificial insemination
collecting sperm cells and manually depositing them into the report system of a female
embryo transfer
implanting a fertilized ovum into a uterus
in vitro fertilization
eggs are fertilized in a dish resulting in embryos used for transfer or frozen for later use
cloning
creation of an organism genetically identical to another using artificial embryo twinning or somatic cell nuclear transfer
stem cell research
changing undifferentiated cells into specialized cells
transgenic animals
carry a foreign gene that has been deliberately inserted into its genome
transgenic advantages
increased growth, disease resistance, improved nutritional quality, increased muscle mass, wool production
transgenic disadvantages
gene has multiple functions, breeding problems, functional disorders, low survival rate, ethics
transgenic methods
embryonic stem cell, pronucleus, retrovirus mediated gene transfer
embryonic stem cell method
transforming ES cells growing in tissue culture with desired DNA
pronucleus method
injecting gene into pronucleus of a fertilized egg
retrovirus method
retroviruses are used as vectors to transfer genetic material into the host cell
transgenic cows
have extra coops of casein gene to increase milk protein
transgenic pigs
fertilizing eggs with sperm cells containing foreign DNA
transgenic sheep and goats
genes from dragline silk were put into a goat to be expressed in the form of milk
gene knockout
1 or more of an organisms genes are made inoperative
transgenic primates
transgene was incorporated into the gremlin and passed on to offspring; possible best model
transgenic chickens
two methods:
- infecting embryos with a viral vector carrying a human gene for a therapeutic protein and promoter sequence that will make protein in the egg white
- transforming rooster sperm with a human gene and appropriate promoters
antibodies
proteins developed by immune system that recognize specific antigens
antigens
react with the products of a specific immune response
myeloma cell
immortal tumor of lymphocytes
monoclonal antibodies
made by fusing myeloma cells with spleen cells to create a hybridoma capable of unlimited growth