ENZYME REGULATION L11

Question 1

What two things are indicative of liver damage?

Answer 1

1. Alanine Transaminase (ALT)

2. Low level of alpha-1 antitrypsin

Question 2

Define Zymogen

Answer 2

Inactive protein precursor that is converted to an enzyme when activated

Question 3

What activates Zymogen from going inactive to active?

Answer 3

Proteolytic cleavage

Question 4

How is Thrombin generated?

Answer 4

From prothrombin by two proteolytic cleavages performed by prothrombin converting complex

Question 5

What consists of prothrombin-converting complex?

Answer 5

Calcium, phospholipids and 2 blood coagulation proteins (factor V and factor X)

Question 6

T/F: The two cleavages from prothrombin to thrombin leas to an irreversible activation of thrombin

Answer 6

True

Question 7

Warfarin, a vitamin K analog, is used to treat what time of disorder?

Answer 7

Thrombosis (blood disorder): blood coagulates too readily

Question 8

How do you inactivate thrombin?

Answer 8

High affinity binding of antithrombin

Question 9

What is alpha1-antitrypsin?

Answer 9

Inhibitor of elastase hence protects tissues

Question 10

What can change the charge of an amino acid?

Answer 10

Phosphorylation & Dephosphorylation

Question 11

What do Competitive Inhibitors NOT alter?

Answer 11

Vmax

Question 12

Noncompetitive inhibitors reduce _______

Answer 12

Vmax/amount of enzyme present