Enzyme Reactions - Temperature

Question 1

What factors affect the rate of enzyme catalysed reactions?

Answer 1

temperature
p.H
enzyme concentration
substrate concentration
inhibitors - competitive and non competitive

Question 2

What does denatured mean?

Answer 2

a permanent change to the active site which means no more E-S complexes are formed

Question 3

What happens during enzyme reactions where temperature plays a role?

Answer 3

1) temperature - as temp. increases, the molecules vibrate more, so more collisions, and a higher % of collisions will have the activation energy - so more E-S complexes are formed
2) optimimum temperature - molecules vibrate and break internal bonds ( ionic + disulphide bridges), causing changes to the shape of active site, and then doesnt form E-S complexes
3) enzyme is denatured