Enzyme Reactions

Question 1

Describe the enzyme reaction for investigating the effect of temperature on rate of reaction.

Answer 1

Independent Variable:
- temperature

Dependent Variable:
- rate of reaction

Control Variables:
- optimum pH (buffer)
- volume and concentration of enzymes/substrates
- time

Control Experiment:
- boil enzyme to denature it
- repeat experiment using smaller intervals

Question 2

Describe the enzyme reaction for investigating the effect of pH on rate of reaction.

Answer 2

Independent Variable:
- pH

Dependent Variable:
- rate of reaction

Control Variables:
- optimum temperature
- volume and concentration of enzymes/substrates
- time

Control Experiment:
- boil enzyme to denature it
- repeat experiment using smaller intervals

Question 3

Describe the enzyme reaction for investigating the effect of substrate concentration on rate of reaction.

Answer 3

Independent Variable:
- substrate concentration

Dependent Variable:
- rate of reaction

Control Variables:
- optimum temperature and pH
- volume and concentration of enzyme
- volume of substrate
- time

Control Experiment:
- boil enzyme to denature it
- repeat experiment using smaller intervals