Enzyme Lab Flashcards
What are enzymes?
Catalyst in biochemical pathways ( protein or nucleic acid : ribosome)
What is catalase ?
Type of enzyme : antioxidant, catalyses ( H2 O2) breakdown by lowering the activation energy. It is an active site that binds to substrate by induced fit
Hydrogen peroxide ( H2 O2) breakdown equation catalyzed by catalase .
2 H2 O2 + catalase —> 2H2O + O2 + cat.
Hydrogen + “ —> water + oxygen + “
Peroxide
( —> enzymes substrate complex)
What affects enzymes ?
What do they affect ?
- Temperature , salinity, PH
- Secondary tertiary and quaternary structures.
How does temperature affect enzymes ?
Affects all bonds
How does PH and salinity affect enzymes?
Affects ionic bonds between R-groups
What is hydrogen peroxide ?
It is a by-product of cell metabolism and can damage the cell if it it left unchecked.
What is the substrate of catalase?
Hydrogen peroxide
What is a optimal condition?
It is condition which are based on specific 3D structures in which the Enzyme will fonction the best.
What happens if Enzymes are exposed to conditions exceeding their optimum ?
Can permanently denature ( lose 3D shape and fonction )
How can enzyme kinetic be measured?
1)Rate of disappearances of H2O2 (substrate)
2) Rate of appearance of O2 (product)
How does PH affect the activity of catalase ?
The more the PH approches to 7, the faster the catalase will react.
How does PH affect the activity of catalase ?
The more the PH approches to 7, the faster the catalase will react.
When do we know a catalysed reaction is saturated?
Once all the active sites are occupied by substrate molecules the reaction rate reaches its maximum value ( V max)
System is saturated because adding more substrate wouldn’t make the reaction go faster. LINE PLATEAUS.
