Enzyme kinetics

Question 1

Effect of competitive inhibitor

Answer 1

increases apparent Km (decreasing enzyme affinity for substrate)
no effect on Vmax
Lineweaver-Burke: increases x-intercept, same y-intercept

Question 2

Competitive inhibitor method of action

Answer 2

binds REVERSIBLY to active site of enzyme, competing with substrate
can be outcompeted! with enough addition of [S]

Question 3

Noncompetitive inhibitor method of action

Answer 3

binds to allosteric site on enzyme

cannot be outcompeted with additional [S]

Question 4

Mechanisms for regulating enzymatic activity

Answer 4

substrate availability
product inhibition
allosteric control
covalent modification
enzyme induction/repression of synthesis