Enzyme Catalysis and Kinetics Flashcards
To understand the Kinetic basis for enzyme catalytic activity.
1
Q
What is the Beer-Lambert Relationship?
A
A = ecl
Where A is a unitless measurement for absorbance, e is the molar extinction coefficient, c is concentration of substance (M), and l is pathlength (cm).
2
Q
Enzyme Activity
A
2 steps: first find the initial velocity of reaction (amount of product formed, divided by length of time to form it). Second, divide the initial velocity by the [enzyme] or the weight concentration of protein to obtain the enzyme activity (per “unit,” enzyme etc) **Use Molar [products]… (divide by volume etc.)
3
Q
What are the 3 types of enzyme inhibition?
A
- Competitive: app Km increases, Vmax/Kcat stays the same. 2. Uncompetitive: Inhibitor binds enzyme before substrate. Kcat decreases and Km decreases 3. Noncompetitive: Inhibitor binds at beginning or after enzyme binds. app Kcat decreases and app Km stays the same.
