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PHEW > Enteric pathogens > Flashcards

Enteric pathogens Flashcards

1
Q

What is the main pathogen causing foodborne illness in the UK?

A

Campylobacter

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2
Q

What culture material is usually used to isolate enteric pathogens?

A

MacConkey agar - contains bile salts

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3
Q

How does E. coli appear on MacConkeys agar?

A

Ferments lactose forming pink/ red colonies

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4
Q

How does Salmonella appear on MacConkeys agar?

A

Does not ferment lactose and therefore agar does not change

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5
Q

Brilliant green again is selective for what bacteria?

A

Salmonella - colour change from yellow to red

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6
Q

EMB media is selective for what bacteria?

A

E.coli

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7
Q

Which media is used to isolate Campylobacter?

A

Blood based agar - CCDA/ Skirrows. Reduced O2 tension

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8
Q

How does phage typing work?

A

Bacteria are tested against a panel of bacteriophages

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9
Q

What definitive methodology is used to type bacteria?

A

Genome sequencing

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10
Q

EPEC

A

Entero pathogenic E. coli

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11
Q

ETEC

A

Entero toxigenic

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12
Q

EIEC

A

Entero Invasiv

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13
Q

EHEC

A

Entero Haemorrhagic

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14
Q

EAEC

A

Entero Aggregative

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15
Q

UPEC E. coli is associated with what antigen and pathology?

A

Urinary tract infection (UPEC) allow attachment to
urethra or bladder.

Pili & Fimbriae enteric forms and UPEC to allow attachment

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16
Q

LPS is associated with what E.coli pathology?

A

Septicaemia

17
Q

Which virulence factor is associated with ETEC?

A

Enterotoxin

18
Q

What virulence factor is responsible for gut attachment and invasion of EPEC and EHEC?

A

T3 secretion system

19
Q

Verotoxin and Shiga-like toxins cause what pathology?

A

Target blood vessels and causing bloody diarrhoea (gut) and haemolytic uremic syndrome (kidney)

20
Q

Salmonella is most commonly found in which foodstuffs?

A

Eggs and meat

Foodstuffs contaminated with faeces from infected animals

21
Q

Salmonella species associated with non-typhoidal salmonellosis

A

Enteritidis

Typhimurium

22
Q

Incubation period of non-typhoidal salmonellosis.

A

12-72 hours

23
Q

Sudden onset of painful abdominal cramps, followed by fever, headache, diarrheoa (sometimes bloody) and vomiting. Symptoms lasting 5 days.

What pathogen may be suspected?

A

Salmonella

24
Q

Which phage type of Salmonella is most commonly found in the UK?

A

4

25
Q

What makes the broiler an ideal incubator for Campylobacter?

A
  • 41-42°C - thermophilic bug
  • Low oxygen tension - microaerophiles
  • High levels of growth in caeca (up to 1010 CFU per g)
  • High levels of shedding in faecal and caecal droppings & coprophagic behaviour
  • Campy persists in the caeca

PHEW (6 decks)

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