energy transfer in and between organisms Flashcards
Location of light dependent reaction
Thylakoid membranes of chloroplast
Location of light independent reaction
Stroma of chloroplast
Thylakoid membranes
Folded membranes containing photosynthetic proteins (chlorophyll)
embedded with transmembrane electron carrier proteins involved in the LDRs
Chlorophyll
Located in proteins on thylakoid membranes
mix of coloured proteins that absorb light
different proportions of each pigment lead to different colours on leaves
Light-dependent reaction (LDR)
First stage of photosynthesis occurs in thylakoid membranes uses light energy and water to create ATP and reduced NADP for LIR
involves photoionisation of chlorophyll, photolysis and chemiosmosis
Advantage of many pigments
Each pigment absorbs a different wavelength of visible light
many pigments maximises spectrum of visible light absorbed
maximum light energy taken in so more photoionisation and higher rate of photosynthesis
Photolysis
Light energy absorbed by chlorophyll splits water into oxygen, H+ and e-
H20 –> 1/2 O2 + 2e- + 2H+
Products of photolysis
H+
Picked up by NADP to form
reduced NADP for LIR
e-
passed along chain of
electron carrier proteins
oxygen
used in respiration or diffuses out leaf via stomata
Chemiosmosis
Electrons that gained energy move along a series of electron carriers in thylakoid membrane release energy as they go along which pumps proteins across thylakoid membrane electrochemical gradient made protons pass back across via ATP synthase enzyme producing ATP down their conc. gradient
Photoionisation of chlorophyll
Light energy absorbed by chlorophyll excites electrons so they move to a higher energy level and leave chlorophyll some of the energy released is used to make ATP and reduced NADP
What happens to protons after chemiosmosis
Combine with co-enzyme NADP to become reduced NADP reduced NADP used in LIR
Products of LDR
ATP (used in LIR)
reduced NADP (used in LIR) oxygen (used in respiration / diffuses out stomata)
Light independent reaction (LIR)
Calvin cycle
uses CO2, reduced NADP and ATP to form hexose sugar occurs in stroma which contains the enzyme Rubisco temperature-sensitive
RuBP
Ribulose Bisphosphate
5-carbon molecule
GP
Glycerate-3-phosphate
3-carbon molecule
TP
Triose phosphate
3-carbon molecule
Producing hexose sugar in LIR
Takes 6 cycles
glucose can join to form disaccharides (sucrose) or polysaccharides (cellulose) can be converted to glycerol to combine with fatty acids to make lipids
Limiting factor
A factor which, if increased, the rate of the overall reaction also increases
Limiting factors of photosynthesis
Light intensity
CO2 concentration
temperature
How light intensity limits photosynthesis
If reduced, levels of ATP and reduced NADP would fall
LDR limited - less photolysis and
photoionisation
GP cannot be reduced to triose phosphate in LIR
How temperature limits photosynthesis
LIR inhibited - enzyme controlled (Rubisco)
up to optimum, more collisions and E-S complexes
above optimum, H-bonds in tertiary structure break, active site changes shape - denatured
How CO2 concentration limits photosynthesis
If reduced, LIR inhibited
less CO2 to combine with RuBP to form GP
less GP reduced to TP
less TP converted to hexose and RuBP regenerated
Products of LIR
Hexose sugar
NADP - used in LDR
Agricultural practices to maximise plant growth
Growing plants under artificial lighting to maximise light intensity
heating in greenhouse to increase temperature
burning fuel to release CO2
Benefit of agricultural practices for plant growth
Faster production of glucose -> faster respiration
more ATP to provide energy for growth e.g. cell division + protein synthesis
higher yields so more profit
Stages of aerobic respiration
1) Glycolysis
2) Link reaction
3) Krebs cycle
4) Oxidative phosphorylation
Location of glycolysis
Cytoplasm
Glycolysis
Substrate level phosphorylation
- 2 ATP molecules add 2 phosphate groups to glucose glucose phosphate splits into two triose phosphate (3C) molecules
both TP molecules are oxidised (reducing NAD) to form 2 pyruvate molecules (3C) releases 4 ATP molecules
Coenzymes
A molecule which aids / assists an enzyme
NAD and FAD in respiration both gain hydrogen to form reduced NAD (NADH) and reduced FAD (FADH)
NADP in photosynthesis gains hydrogen to form reduced NADP (NADPH)
Products of glycolysis
Net gain of 2 ATP
2 reduced NAD
2 pyruvate molecules
How many ATP molecules does glycolysis produce
2 ATP molecules used to phosphorylate glycose to glucose phosphate
4 molecules generated in oxidation of TP to pyruvate net gain 2 ATP molecules
Location of the link reaction
Mitochondrial matrix
Link reaction
Reduced NAD and pyruvate are actively transported to matrix
pyruvate is oxidised to acetate (forming reduced NAD)
carbon removed and CO2 forms
acetate combines with coenzyme A to form acetylcoenzyme A (2C)
Products of the link reaction per glucose molecule
2 acetylcoenzyme A molecules 2 carbon dioxide molecules released
2 reduced NAD molecules
Mitochondria structure
Double membrane with inner membrane folded into cristae enzymes in matrix
Location of the Krebs cycle
Mitochondrial matrix