CLASSIFICATION + DIVERSITY Flashcards

TOPIC 4

1
Q

GENE

A

section of DNA that contains code for making polypeptide + functional RNA

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2
Q

LOCUS

A

location of particular gene on chromosome

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3
Q

ALLELE

A

location of particular gene on chromosome

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4
Q

CHROMOSOME

A

threadlike structure composed of tightly coiled DNA + wrapped around histones

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5
Q

HOMOGLOUS CHROMOSOMES

A

pair of chromosomes that have same genes therefore- same size

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6
Q

PROKARYOTIC DNA

A

Shorter
Circular
Not associated w/histones
No nucleus
May have plasmids
Only exons

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7
Q

EUKARYOTIC DNA

A

Longer
Linear
Associated w/ histones
Contains nucleus
No plasmids
Have introns + exons

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8
Q

CODON

A

3 bases on mRNA- code for amino acid

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9
Q

START CODON

A

3 bases at start of mRNA sequence which help initiate translation

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10
Q

STOP CODON

A

3 bases at end of every gene that don’t code for amino acid- ribosomes to detach + therefore stops translation

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11
Q

UNIVERSAL

A

code universal for all living things

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12
Q

GENETIC CODE

A

amino acid is coded for by 3 DNA bases which- described as “triplet code”

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13
Q

ADVANTAGE OF THE CODE BEING UNIVERSAL

A

genetic engineering is possible human gene can be inserted into another organism
e.g human gene for insulin inserted to bacteria to make insulin

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14
Q

NON-OVERLAPPING

A

each triplet is read separately

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15
Q

ADVANTAGE OF THE CODE BEING NON-OVERLAPPING

A

if point mutation occurs, it will only affect one codon and therefore one amino acid

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16
Q

DEGENRATE

A

each amino acid is coded for by
more than one triplet of bases

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17
Q

ADVANTAGE OF THE CODE BEING DEGENERATE

A

if substitution mutation occurs new triplet of bases may still code for same amino acid
therefore mutation will have no impact on final protein produced

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18
Q

MUTATION

A

change in DNA can be gene or chromosome mutation

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19
Q

INTRON

A

non-coding sequence of DNA

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20
Q

EXON

A

sequences in gene that code for amino acid sequences

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21
Q

MRNA

A

.Single stranded
.Linear chain
.Longer chain/ has more nucleotides
.Corresponds to genetic sequence of gene
.Unpaired bases

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22
Q

MRNA FUNCTION

A

copy of gene from DNA created in nucleus, and it then leaves nucleus to carry copy of genetic code of one gene to ribosome in cytoplasm

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23
Q

TRNA

A

.Single stranded folded into a clover shape
.Hydrogen bonds
.Anticodon
.Amino acid attachment site
.All similar lengths

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24
Q

TRNA FUNCTION

A

single-stranded, folded to create cloverleaf shape held in place by hydrogen bonds
has an anticodon and amino acid binding site

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25
GENEOME
complete set of genes in cell
26
PROTEOME
full range of proteins that cell is able to produce
27
POLYPEPTIDE SYNTHESIS
.sequence of nucleotides on gene on DNA acts as template .complimentary copy is transcribed onto mRNA in nucleus .At ribosomes mRNA acts as template that is translated to chain of amino acids using complementary tRNA which carry specific amino acids which are linked together
28
TRANSCRIPTION FROM DNA TO MRNA
1.H bonds between complementary base pairs of gene to be transcribed are broken DNA nucleotide bases are now exposed 2.One strand acts as template to make an mRNA copy 3.Free RNA nucleotides alongside their complementary base pair on template strand of DNA-Uracil replaces thymine on mRNA 4.RNA polymerase joins adjacent nucleotides together w/phosphodiester bond 5.RNA Polymerase continues until it reaches stop signal and then it detaches + introns are removed
29
PRE-MRNA + SPLICING
.eukaryotic cells, mRNA produced during transcription contains both introns and exons-this is pre-mRNA .introns need to be removed because they don’t contain any genetic information that can be translated into an amino acid sequence .introns are “spliced” out of mRNA sequence by an enzyme .produces strand of mRNA that contains only exons - coding regions- known as mature mRNA
30
TRANSLATION OF MRNA TO FORM POLYPEPTIDE
1. mRNA attaches to ribosomes 2. tRNA anticodons bind to complementary mRNA codons 3. tRNA brings a specific amino acid 4. Amino acids join by peptide bonds 5. use of ATP 6. tRNA released after amino acid joined to polypeptide 7. ribosome moves along mRNA to form polypeptide
31
HAPLOID
one copy of each chromosome in cell
32
DIPLOID
two copies of each chromosome in cell
33
MEOSIS
Meiosis I is reduction division separation of homologous pair of chromosomes: .Homologous pairs are separated .Variation is introduced Meiosis II is similar to mitosis w/separation of sister chromatids Four haploid cells are produced, each w/ half diploid number of chromosomes
34
CROSSING OVER
occurs in prophase I w/ formation of chiasmata 1.Homologous pairs of chromosomes associate and form bivalent 2.Chiasmata forms 3.Equal lengths of non-sister chromatids or alleles are exchanged 4.Producing new combinations of alleles
35
INDEPENDANT SEGREGATION
1.Metaphase 1, homologous pairs line up either side of equator 2.completely random which side of equator maternal and paternal chromosomes line up on 3.When separated, one of each pair ends up in daughter cell 4.‘shuffling’ leads to different combinations of maternal and paternal chromosomes in daughter cells
36
OTHER SOURCES OF VARIATION
.Fertilisation: random mating w/random gametes –results in random fusion of gametes and produces new allele combinations/new maternal and paternal chromosome combinations .mutations may occur
37
INSERTION
extra base is added during DNA replication results in frame shift – all codons after mutation are affected
37
MITOSIS VS MEOISIS
Same number of chromosomes VS Half number of chromosomes Genetically identical VS Genetically different Two cells VS Four cells
38
DELETION
One base is left out during DNA replication results in frame shift – all codons after mutation are affected
38
SUBSITUTION
One base is changed for another during DNA replication-Only codon w/mutation is affected
39
CHANGE IN CODON RESULTS CHANGE IN PROTEIN
.amino acid sequence changes .hydrogen/ionic/disulphide bonds between R groups changes .tertiary structure changes
40
NON-FUNCTIONAL ENZYME
change is in active site of an enzyme then active site is no longer complementary to substrate and enzyme cannot form enzyme-substrate complexes
41
MUTAGENIC AGENTS
increase rate of gene mutation eg. UV radiation
42
CHROMSOME MUTATION
.Most likely to occur in meiosis – not being shared equally between daughter cells in metaphase and anaphase .Changes in numbers: Non-dysjunction where one daughter cell gets both of homologous pair and other none
43
DEFINE GENE MUTATION + EXPLAIN HOW GENR MUTATION CAN HAVE NO EFFECT OR POSITIVE EFFECT ON AN INDIVIDUAL
1. change in base sequence 2. formation of new allele NO EFFECT 3. genetic code is degenerate 4. does change amino acid but no effect on tertiary structure 5. recessive so doesn't influence phenotype POSITIVE EFFECT 6. change in polypeptide that positively changes protein 7. may result in increased reproductive success
44
THINGS THAT AFFECT GENETIC DIVERSITY OF POPULATION
1. Population size – smaller populations generally have lower genetic diversity and larger populations larger one 2. Inbreeding – reduces genetic diversity 3. Migration – increases genetic diversity 4. Mutations – increases genetic diversity 5. Genetic Bottlenecks – reduces genetic diversity 6. Founder effect – reduces genetic diversity 7. Natural selection – increases frequency of beneficial alleles
45
BOTTLENECK EFFECT
previously large population suffers dramatic fall in numbers major environmental event can massively reduce number of individuals in population which in turn reduces genetic diversity in population as alleles are lost surviving individuals end up breeding and reproducing w/close relatives
45
FOUNDER EFFECT
only small number of individuals from large parent population start new population new population is made up of only few individuals from original population only some of total alleles from parent population will be present not all of gene pool is present in smaller population gene pool is complete range of DNA sequences that exist in all individuals of population or species
46
NATURAL SELECTION
.random mutation result in new alleles of gene .selection pressures exist in environment .increase/decrease in chance of survival and reproduction .advantageous alleles pass on to offspring .many generations-increasing allele frequency in population and change in gene pool
47
DIRECTIONAL SELECTION
.Individuals w/alleles for an extreme type are more likely to survive and reproduce .Some bacteria have alleles that give them resistance to an antibiotic .normal population of bacteria are killed by antibiotic .Resistant bacteria survive and reproduce w/X competition passing on allele for antibiotic resistance to their offspring .After some time most of population will have antibiotic resistance allele
48
STABILISING SELECTION
.Individuals w/alleles for middle of the range are more likely to survive and reproduce .Small babies find it hard to maintain their body temperature so less likely to survive .Large babies less likely to survive also .So conditions most favourable for medium sized babies .weight of human babies shifts towards middle of range
49
HOW NATURAL SELECTION BETTER ADAPTS SPECIES
.Anatomical adaptations: Structural features eg. whales have thick layer of blubber to keep warm in cold sea .Physiological adaptations: Processes in organism eg. Hibernation over winter reducing metabolic rate to conserve energy when food is scarce .Behavioural adaptations: way an organism acts eg. possums playing dead near to predators
50
RP6-USING ASEPTIC TECHNIQUES
.Set up petri dish w/nutrient agar .Use sterile technique to transfer bacteria from liquid broth to agar .Spread bacteria over plate using spreader .Use sterile forceps to place paper discs soaked w/different concentrations of antibiotic .Also add negative control disc just soaked in sterile water .Incubate at 25oC for 48 hours Inhibition zones are clear: Anywhere bacteria can not grow .size of inhibition zone is how well antibiotic has grown
51
RP6-TYPES OF ASEPTIC TECHNIQUES
.Avoids contamination of cultures from unwanted microorganisms, particularly disease causing .Heat glassware, petri dishes and agar in an autoclave in steam under pressure at 121oC for 15 minutes .Sterilise work surfaces before and after using disinfectant .Use Bunsen to set up an air flow upwards to carry bacteria away .Lift petri dish lids w/just enough angle to allow entry of loop and for short time .Secure petri dish lids w/ 2 pieces of tape .Sterilise plastic material using gamma radiation
52
PHYLOGENETIC CLASSIFICATION
attempts to arrange species to groups based on their evolutionary origins and relationships
53
HIERACRCHY
Smaller groups are placed in larger groups w/no overlap between groups Domain → kingdom → phylum → class → order → family → genus → species
54
BINOMAIL SYSTEM
genus and species name
54
PROBLEMS W/USING OBSERVABLE FEATURES IN CLASSIFICATION
Some organisms will look alike due to convergent evolution 2 organisms evolve similar features but are not closely related live in similar environments and are exposed to similar selection pressures Similar alleles will provide similar selective advantages
55
ADVANCES TECHNIQUES CAN HELP TO CLARIFY EVOLUTIONARY RELATIONSHIPS BETWEEN ORGANISMS
.DNA sequences – more similarities between base sequences more closely related organisms are e.g same genus .Amino acid sequences – more similarities in amino acid sequence more closely related organisms are .Immunology- Similar proteins in organisms will bind same antibodies eg. see if antibodies that bind to human version of protein will also bind to mouse version of protein
56
PHYLOGENETIC TREE
Phylogeny is study of evolutionary history of groups of organisms It tells us who’s related to who and how closely Phylogenetic trees show these evolutional relationships Each branch is common ancestor
56
BIODIVERSITY
variety of organisms living in an area relate to range of habitats from small local habitats eg. pond measure of species richness and abundance
57
COURTSHIP BEHAVIOUR
organisms to attract mate of right species essential for successful mating so that organisms can pass on advantageous alleles and species can survive It could be releasing chemicals or series of displays Behaviours and actions which are unique to each species- allows species identify individuals of their own species to mate and reproduce w/ normally performed by males eg. dance, song+ Females choose individual they wish to mate w/ Courtship ensures successful reproduction - Enables individuals to recognise others of their own species and individuals of opposite sex- also synchronises mating behaviour – mating occurs only when individual is sexually mature and in season Courtship increases survival of offspring - Formation of pair bonds – 2 parents looking after offspring increases its survival
58
COMMUNITY
all species in particular area at particular time
58
HABITAT
range of physical, biological and environmental factors in which species can live
59
SPECIES RICHNESS
measure of number of different species in community It can be calculated by taking random samples of community and counting number of different species
60
INDEX OF DIVERSITY EQUATION
d=N(N-1)/n(n-1) .N = total number of organisms of all species .n = total number of organisms of each species .higher number d, more diverse an area is
61
AGRICULTURE
cultivation of soil to grow crops usually involves clearing of land to allow more space for crop growing- It often includes use of herbicides to kill weeds and pesticides to kill insects which may feed off of crops-These practices reduce biodiversity of areas
62
WHY REMOVING WOODLANDS REDUCES BIODIVERSITY
Less habitats, less variety of food sources
63
WHY USING PESTICIDES REDUCES BIODIVERSITY
Kills insect species- reduction in variety of food sources for organisms higher up food chain
64
WHY USING HERBICIDES REDUCES BIODIVERSITY
Kills species of weeds- reduction in variety of food types for herbivores
65
WHY GROWING 1 TYPE OF CROP REDUCES BIODIVERSITY
Less variety in food sources- less variety of habitats
66
CONSERVATION
balance between need for agriculture and maintaining biodiversity
66
GENE TECHNOLOGY CAUSING CHANGE IN METHODS OF INVESTIGATING GENETIC DIVERSITY
.Inferring DNA differences from measurable or observable characteristics has been replaced by direct investigation of DNA sequences
67
STUDENT T-TEST
comparing mean values of two data sets
67
INTERPRETING DATA RELATING ON DNA BASE SEQUENCES + AMINO ACID SEQUENCES
.Suggest relationships between different organisms in species and between species .More similarities mean that organisms are more closely related
68
GENE TECHNOLOGY ALLOWING GENETIC DIVERSITY IN OR BETWEEN SPECIES TO BE INVESTIGATED
.By comparing base sequence of DNA .By comparing base sequence of mRNA .By comparing amino acid sequence of proteins
69
COLLECTING DATA FROM RANDOM SAMPLES OF POPULATION
.Divide area into grid .use random computer number generator .use large number of samples to collect reliable and representative sample