Energy for Processes Flashcards

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1
Q

describe the structure of a chloroplast

A

usually disc shaped
double membrane
thylakoids-flattened discs stack to form grana
integral lamellae -tubular extensions attach thylakoids to adjacent grana
stroma- fluid filled matrix with high enzyme and substrate conc

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2
Q

where do the light dependent and light independent stages occur

A

light dependent-thylakoids
light independent -stroma

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3
Q

what is the role of photosynthetic pigments

A

absorb wavelengths of light

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4
Q

name the two main groups of photosynthetic pigments

A

primary pigment-chlorophyll found in photosystems
accessory pigments-carotenoids found in light harvesting systems

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5
Q

name the processes in the light dependent reaction

A

photoionisation
electron transfer chain
chemiosmosis

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6
Q

explain the role of light in photoionisation

A

chlorophyll molecules absorb energy from photons in light
this excites electrons raising them to higher energy level
causes them to be released from chlorophyll

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7
Q

what happens in the electron transfer chain

A

electrons released from chlorophyll move down a series of carrier proteins embedded in thylakoid membrane
undergo redox reactions which release energy

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8
Q

how does chemiosmosis produce ATP in light dependent stage

A

some energy released from ETC is coupled to active transport of H+ ions from stroma into thylakoid space
H+ ions move down concentration gradient into stroma by transmembrane channel protein ATP synthase
ATP synthase catalyses ADP+Pi–> ATP

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9
Q

describe non-cyclic photophosphorylation

A

uses photosystems 1 and 2
excited electrons enter ETC to produce ATP
NADP acts as final electron acceptor and is reduced
water is photolysed to release electrons to replace those lost in photosystem 2
purpose is to produce ATP and reduced NADP for Calvin cycle

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10
Q

describe cyclic photophosphorylation

A

uses only photosystem 1
excited electrons enter ETC to produce ATP then return directly to photosystem
purpose to produce additional ATP

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11
Q

what happens in photolysis of water

A

light splits water molecules

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12
Q

what happens to the products of the photolysis of water

A

H+ ions-move out of thylakoid space by ATP synthase and used to reduce NADP
e- -replace electrons lost from chlorophyll
O2-used for respiration

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13
Q

name the 3 main stages in the light independent reaction

A

carbon fixation
reduction
regeneration

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14
Q

what happens during carbon fixation

A

reaction between CO2 and RuBP catalysed by RuBisCo
forms unstable 6 carbon compound that breaks down into 2 glycerate 3 phosphate (GP)

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15
Q

what happens during reduction in the light independent stage

A

2 GP reduced to 2 triose phosphate
requires 2 reduced NADP and 2 ATP
forms 2 NADP and 2 ADP

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16
Q

outline the roles of TP from the light independent reaction

A

1 carbon leaves to produce monosaccharides, amino acids and other biological nutrients
involved in regeneration of RuBP
after 1 carbon leaves, 5 carbon compound RuP forms
converted into RuBP with 1 ATP

17
Q

state the number of carbon atoms in RuBP, GP and GALP

A

RuBP=5
GP=3
GALP=3

18
Q

define limiting factor

A

factor that determines maximum rate of a reaction even if other factors change to become more favourable

19
Q

name 4 environmental factors that can limit the rate of photosynthesis

A

light intensity (light dependent)
CO2 concentration (light independent)
mineral levels-maintain normal functioning of chlorophyll
temperature-enzyme stages

20
Q

how does light intensity affect the rate of photosynthesis

A

low light intensity-slower light dependent reaction
less ATP and NADP produced to convert to GP and TP

21
Q

describe the implications of water stress

A

abscisic acid binds to complementary receptors on guard cell membrane-ca2+ channels open
positive feedback triggers other ion channels to open
water potential of guard cells becomes more positive-water diffuses out by osmosis
guard cells become flacid so stomata close

22
Q

state the purpose and principle of paper chromatography

A

molecules in a mixture are separated based on relevant attraction to mobile phase vs stationary phase

23
Q

outline a method for extracting photosynthetic pigments

A

use a pestle and mortar to grind a leaf with an extraction solvent

24
Q

outline how TLC can be used to separate photosynthetic pigments

A

use capillary tube to spot pigment extract onto pencil line
place chromatography paper in solvent
allow solvent to run until it almost touches other end of paper