EMRCS Flashcards
Tissue Processing Steps
1) Place specimen in vessel for transport
2) Clear labelling with patient details + provision of clinical details
3) Treatment with saline/formalin
4) Tissue Fixing
5) Impregnation with wax
6) Microtome cutting / embedding
7) Staining - H&E + Immunohystochemistry
Method of assessment for intraoperative tissue assessment
1) Call pathology department to warn of frozen section
2) Tissue taken to pathology department
3) Tissue cut
4) Frozen in liquid nitrogen
5) Cut using cryostat
6) Staining
7) Examined by pathologist
Why might cytology not be adequate for assessment of disease?
Architectural elements of certain pathologies are important:
Capsule Breach
Basement membrane intact
Formation of psuedocysts
Formalin:
- Frozen Section or Embedded Section?
- How does it work?
Formalin Fixation is used in the Embedded Section process - this is not used intraoepratively/ in emergency
- Used in conjunction with wax and in general is better in terms of quality than Frozen sections
- time consuming process
Works by causing crosslinking of collagen fibres maintaing architectural integrity of samples.
Clostridium Difficile
- Basic Bacteriology
- Toxins
- What facilitates its transmission
- What is a spore
- Risk Factors
- Drugs used for treatment of C Diff
- Treatment of Toxic Megacolon
Gram Positive, Spore Forming Rod - Facultative Anaerobe
Toxins - Enterotoxin A and Cytotoxin B
Spread - is conferred through highly resistant spores
Spore - spores are one cell organisms capable of giving rise to new individual organisms without sexual fusion
Risk Factors - Antibiotics, Anti Acids, Transmission
Treatment - Metronidazole PO, Vancomycin PO, Fidoxamicin
Toxic Megacolon - Resuscitate patient. Usually no place for further antibiotics. Laparatomy - Subtotal Colectomy + End Ileostomy Formation
Factors Reducing the rate of SSIs:
6
Do wound protectors reduce the risk?
1) Not shaving
2) Good sterility practice
3) Perioperative/Postoperative antibiotics
4) Skin Prep
5) Not using incise drapes - Non idoine drape if necessary
6) Use a knife rather than diathermy for skin incision
Wound protectors have not demonstrated reduced risk
Key acute inflammation features
1) Vasodilation
2) Protein Rich Exudate
3) Neutrophils/Mast Cells –> Macrophages/Lymphocytes
Why do abscesses form?
1) High Microbial Load at site
2) Anatomical Factors Causing confinement of the exudate
3) Hypoxic core of abscess leads to imedence of the normal pathogen eradication mechanisms (Free Radicals, phagocytosis)
Definition:
Empyema
Histological Diagnostic Feature of acute inflammation
Metaplasia
Fournier’s Gangrene
Empyema - Formation of pus in a hollow viscus
Presence of neutrophils are a histological diagnostic feature of acute inflammation
Metaplasia - Transformation of specialised epithelium to another
Fourniere’s Gangrene - Synergistic infection - necrotising fascitis of the scrotal area
Sequelae of acute inflammation (4)
Resolution
Organisation - Fibrosis formation
Suppuration - Formation of abscess/empyema
Chronic Inflammation - Balance between inflammation and reparation
Amyloid Protein Types:
Most common sites (2)
Diagnosis
AL - Myeloma
AA - Chronic Inflammatory Conditions (rhemuatoid, ank spond)
Common Sites for amyloid Deposition:
Heart and Kidney
Diagnosis:
Congo Red Stain
Birefringence of polarised light
Barrett’s histology
Worry with finding of dysplasia in sample
Treatment options for severe dysplasia as a finding
Specifics regarding biopsies
Treatment of barratt’s
Barrett’s:
Change from squamous cell in oesophagus to columnar epithelium + goblet cells
Dysplasia Finding in biopsy:
May be the case that there is a missed foci of cancer that has not been picked up
Severe Dysplasia Treatments:
Photodynamic therapy, Endoscopic submucosal resection, lasar ablation + Segmental Resection
Biopsies should be :
Quadrantic and at 2-3 cm intervals/ Time interval 2 -5 years
Barratt’s Treatment:
PPI, Lifestyle modification.
Regular Endoscopy
Consider pH and manometry - ?Anti GORD procedure
Altered Tissue Perfusion, Sepsis and Cutaneous Anaesthesia
Cause
Types
RFs
Mx
Necrotising Fascitis - Altered Tissue Perfusion, Sepsis and Cutaneous Anaesthesia
Cause-Sometimes trauma. Mixed Antibiotics (Group A Strep + Anaerobes)
Types - I - Polymicrobial, II- Group A Strep, III - Clostridium Perfringens
RFs - Immunosuppresion, PAD, DM
Mx - Investigations Resusc, Broad Spec Antibiotics, Surgical Debridement (Wide Debridement, return to theatre every 24o
- Internal Oblique
- Anterior Rectus Sheath
- Rectus Abdominis
- Transversus Aponeurosis
- Conjoint tendon
- Inguinal Ligament
- Spermatic Cord
- External Spermatic Fascia
- Ilioinguinal Nerve
Define :
1) Borders of inguinal canal
2) What is the inguinal canal
3) What is the Superficial ring
4) What is the deep ring
5) Spigellian Hernia
6) Borders of hasselbach’s triangle
1) Inguinal Canal:
Anterior - External oblique aponeurosis
Roof - Transversalis Fascia, Internal Oblique + Transversus Abdominus
Floor- Inguinal Ligament (continuous with external oblique aponeurosis) + Lacunar Ligament (medially)
Posterior - Transversalis Fascia
2) Inguinal canal:
Oblique muscular canal connecting the preperitoneal space at the deep ring to the superficial ring beneath the skin at the pubis
3) Superficial Ring is a defect in the inguinal ligament (EO aponeurosis) medial to pubic tubercle
4) Deep ring is a defect in the transversalis fascia 1cm above amove midpoint of inguinal ligament
5) Spigellian hernia:
At the level of arcuate line. Hernia between:
Laterally - Semilunar line and
Medially - Lateral rectal muscle
Through- spigellian fascia
6) hasselbach’s triangle
Medial - Lateral border of rectus muscle
Lateral - Inferior epigastric vessels
Inferior - Inguinal ligament
Rectus Abdominus:
1) Origin
2) Insertion
3) Two bands divided by
4) innervation
Rectus Abdominus
1) Origin - Xiphoid process, 5th, 6th, 7th Costal cartilages
2) Insertion - Pubic Crest, symphysis, tubercle
3) Two bands divided by linea alba
4) innervated by - Lower 6th Thoracic Nerves
External Oblique
1) Origin
2) Insertion
3) innervation
External Oblique
1) Origin - 5th-12th Rib
2) Inserts - Linea ALba, pubic crest, pubic tubercle, anterior half of iliac crest
3) Innervated by lower 6th thoracic nerve
Internal Oblique
1) Origin
2) Insertion
3) Innervation (as well as transversus abdominus)
Internal Oblique
1) Origin - lumbar fascia, iliac crest, inguinal ligament
2) insertion - linea alba, and pubic crest
3) Innervated by lower 6th thoracic nerves, iliohypogastric nerve + ilioinguinal nerves
Contents of inguinal canal
Contents of spermatic cord
Inguinal Canal
Spermatic Cord (male)
Round ligament (female)
+
Ilioinguinal Nerve
Spermatic Cord (Four Threes)
Fascia - External spermatic (EO aponeurosis), cremasteric (IO Aponeurosis), internal spermatic (Transv. Fascia)
Artery- Testicular, Cremasteric, Artery of vas
Nerves- Genitofemoral (genital branch), Ilioinguinal, Sympathetic nerves
Others- Vas deferens, Pampiniform venous plexus, Lymph
Methods of inguinal hernia repair:
6
1) Laparoscipic (TAP or TEP)
2) Lichtenstein mesh repair - reinforce transversalis fascia
3) Mesh plug - occludes the deep ring
4) Bassini Repair - Opposes conjoint tendon of the medial posterior wall of inguinal canal (aponeurosis of IO and TA muscles) and the inguinal ligament
5) Darn repair - sutures between the conjoint tendon and inguinal ligament
6) Shouldice Repair - open inguinal canal and running sutures approximating the musclular/fascial components
- Rectus Abdominus
- Transversus Aponeurosis
- Peritoneum
- Inferior Epigastric Artery
- Testicular Artery
- Artery of vas
- Ductus deferens
- Pampiniform Plexus
- Internal Spermatic Fascia.
- External Spermatic Fascia
- Testes.
- Tail of epididymis
Definitions:
Femoral Canal
Femoral ring/canal boundaries
Femoral Triangle boundaries
Contents of femoral triangle
Hunter’s Canal Borders
Hunter’s Canal contents
1) Femoral Canal is the medial compartment of the femoral sheath that opens into abdomen via femoral ring - contains Cloquet’s LN and Lymph vessels
2) Fem Ring Boundaries:
Ant - Inguinal Ligament
Post - pectineal ligament
Medial - Lacunar Ligament
Laterally - Femoral Vein
3) Femoral Triangle:
Lateral - Sartorius
Medial - Adductor Longus
Superior - inguinal ligament
Floor - pectineus, adductor longus, illiopsoas
Roof - Fascia Lata
4) Contents of femoral triangle:
Femoral nerve, sheath, artery, vein (great saphenous + deep femoral), deep inguinal lymph nodes
5) Hunter’s Canal (tunnel from femoral triangle to opening in adductor magnus [hiatus])
Anteriorly - Sartoritus
Posteromedially - adductor longus and magnus
laterally - vastus medialis
6) Contents of hunter’s canal
Femoral artery, vein, saphenous nerve, nerve to vastus medialis
Inguinal vs Femoral hernias
Palpation
Strangulation
Structures at risk in fem hern surgery
Palpation :
Inguin. - above and medial to Pubic Tub.
Fem. - below and lateral to pubic tub.
Strangulation:
Fem - more likely to strangulate as canal is narrow + lacunar ligament is robust and sharp
Danger to Femoral vein + abhorrent obturator artery in fem hern. surgery
- ASIS
- Pubic Tubercle
- Inguinal ligament
- Fem Vein
- Fem ARtery
- Fem Nerve
- Femoral Branc. of Genitofemoral nerve
- Sup. Ext. Pudendal Artery
- Great Saphenous Vein.
- Lat. Circ. Artery (Ascending)
- Lat. Circ. Artery Proper
- Intermediate Cutaneous nerve of thigh
- Psoas Major
- Iliacus
- Sartorius
- Pectineus
- Adductor Longus
Femoral Artery
Branches
1- suprainguinal
5 - infrainguinal
Suprainguinal (before inguinal ligament)
Inferior epigastric artery
infrainguinal
Superficial External Pudendal
Deep external pudendal
Superficial Circumflex iliac
Superficial epigastric
Profunda femoris artery - after which CFA continues as SFA. Profunda femoris itself gives off medial and lateral circumflex arteries
Emryological Divisions of gut and their blood supplies
Foregut - to Major duodenal pappilla Coeliac Trunk
Midgut - From major duodenal papilla to 2/3 transverse colon SMA
Hindgut - to ectodermal anal canal - IMA
1-9
1 - Aorta 2- IMA 3- CIA 4 - EIA
5 - Superior Rectal Art. 6 - Gonadal Artery
7 - Inferior Vena Cava 8 - Left Common Iliac Vein 9 - Portal Vein