Embryology 1 - Fertilization to Gastrulation Flashcards
Female haploid gametes
oocytes
Where oocytes, progesterone, and estrogen are produced?
Ovaries
The site that receives oocyte from ovaries and where sperm fertilizes the oocyte?
Fallopian tube (in the ampulla)
Site where embryo, placenta, and membranes develop?
Uterus
When does crossing-over occur to mix the maternal and paternal chromatids (genes)?
Prophase 1
When is meiosis completed in an oocyte? When is fertilization completed?
When the sperm penetrates the oocyte; when spermatic pronucleus and oocyte pronucleus fuse => diploid cell (zygote) produced
Person with ovaries is born with a certain number of _______ oocytes that have been paused during the first stage of meiosis and do not continue until ______.
diploid; after puberty
Release of a secondary oocyte from the ovarian follicle
Ovulation
Ovulated secondary oocyte together with zona pellucida is externally covered in…?
Cumulus oophorus = granulosa cells that will rearrange and form corona radiata
What happens when sperm cells penetrate the zona pellucida?
Inject their genetic material into the oocyte
Why can’t double fertilization occur?
After a sperm cell penetrates the zona pellucida, it becomes impenetrable to other sperm cells
What reaction does contact with the zona pellucida trigger for the sperm cells?
Acrosome reaction = sperm secretes digestive enzymes that break down zona pellucida
Protein coat that surrounds an oocyte as well as the early embryo
Zona pellucida
Multicellular organism prior to fetal stage
Embryo
Cells formed during development that do not become part of the neonatal organism but involute or contribute to the fetal membranes (often contribute to the placenta)
Extraembryonic
Approximately 16-cell stage of an embryo with NO BLASTOCOEL
Morula
A spherical mass of cells that is composed of a trophoblast that surrounds a blastocoel and an embryoblast
Blastocyst
A cell that is totipotential and is present during very early development (product of cleavage)
Blastomere
What does totipotential mean?
A cell that can become any cell
What is is called when an embryo becomes surrounded by the endometrium of the uterus?
Implantation
Four structures of an embryo seen around days 4-5? What is the stage called when these 4 structures are present?
Trophblast - layer of cells outside the sphere
Zona pellucida - covering trophoblast
Embryoblast - inner cell mass
Blastocoel - fluid filled cavity
This is the blastocyst stage
What does progesterone level increase cause?
Increased ciliary movement to move the zygote
After 5 days, where does the blastocyst arrive?
Fundus of the uterus
Roles of zona pellucida in early development? (6)
BPPSPP
- Barrier so only one sperm penetrates
- Porous for communication between embryo and maternal rep. structures
- Protects embryo
- Signal to help trophoblast differentiation
- Prevent premature implantation
- Prevent the blastomeres for dissociating
What can early implantation result in?
Ectopic pregnancy
What day will the embryo hatch out of the zona pellucida?
Day 6
What does the endometrial epithelium express?
Different types of mucin proteins and pinopods
What are pinopods?
Small apical processes that contact the blastocyst and helps it adhere to endometrial epithelium
What is blastocyst adhesion mediated by?
Selectin and integrin binding (like leukocyte emigration from the bloodstream)
Once the trophoblast contacts the endometrial epithelium and invades, what happens?
Forms two layers
- Cytotrophoblast - inner
- Syncytiotrophoblast - outer
_______ develops into a multinuclear cell “mass” where the borders between individual cells are indistinct
Syncytiotrophoblast