Electrophoresis Flashcards
1
Q
What is electrophoresis?
A
using electricity to separate DNA fragments by length
2
Q
What is the charge of DNA?
A
negatively charged
3
Q
What do you attach to the DNA fragments?
A
a fluorescent label or stain DNA
4
Q
What do you put DNA fragments into?
A
the well at negative end
5
Q
What do you turn on?
A
current
6
Q
What are the DNA fragments attracted to?
A
positive electrode
7
Q
What moves faster?
A
small fragments
8
Q
What will look the same?
A
all of the DNA fragments no matter how many there are
9
Q
What will look the same?
A
all of the DNA fragments no matter how many there are
10
Q
If there are 2 cuts made, how many DNA fragments are there?
A
3
11
Q
What do you calibrate the scale by?
A
using pieces of DNA of known length
