Electron Transport, Oxidative Phosphorylation, Chemiosmosis

Question 1

After Krebs cycle (electron transport chain) photo

Answer 1

1) Energy from NADH & FADH2: proton motor force
2) NADH & FADH2 —> inner membrane of mitochondrion (electron transport chain)
3) electron transport chain: series of inner membrane proteins (series of redox rxn)

Question 2

Electron transport chain (series of redox rxn)

Answer 2

1) NADH transfer electrons to enzyme NADH dehydrogenase
2) redox rxn —> E
3) E —> enzyme pump 1 proton (H+) into inter membrane space

4) quinone transport electron to next complex (bc1 complex)- same rxn
5) FADH2 enters chain
6) cytochrome c carries e- from bc1 to cytochrome oxidase complex- same rxn

Question 3

Protons pumped out

Answer 3

Protons re-enter mitochondrial matrix- protein ATP synthase (cuz concentration gradient)
Turns 3 times—> 1 ATP

Aerobic respiration: used e- + re- entered protons + oxygen —-> water
Rid of extra proton in mitochondrial matrix, maintain high proton gradient in intermembrane space
Anaerobic : proton + e- + not oxygen element

ADP + phosphate + E from transport
—> ATP (chemiosmosis)

Oxidative phosphorylation: electron transport chain + chemiosmosis

Question 4

Recap, yield

Answer 4

Chemiosmosis: 1 glucose = 32 ATP 
2 NADH (glycolysis) needs 2 ATP for transport to mitochondrion 

Net ATP for 1 glucose (chemiosmosis): 30 ATP