Electron Transport Chain Flashcards
What does glycolysis produce?
- 2 ATP
- Pyruvate
- 2 NADH
How many molecules of NADH are made from a single Acetyl CoA molecule?
3 NADH (from 1 pyruvate)
What is produced from one Acetyl CoA molecule?
- 3x NADH
- FADH2
- GTP
How many molecules of NADH are involved in the ETC?
8 overall
- 2 NADH from glycolysis
- 3 NADH from 1st Acetyl CoA
- 3 NADH from 2nd Acetyl CoA
How are the 2 molecules of NADH produced from glycolysis transfered into the mitochondria?
Malate shuttle
NADH transfers its electrons to OAA to make malate (then becomes NAD+)
Malate crosses mitochndrial membrane then is transfered into OAA (with NAD+ becoming NADH)
What is oxaloacetate converted into which can then allow it to cross the mitochondrial membrane?
Aspartate (via Glut -> a-KG)
What is aspartate converted back into in the cytosol after crossing the mitochondrial membrane?
OAA (via a-KG -> Glut)
What enzyme converts Dihydroxyacetone phosphate to Glycerol Phosphate (via NADH -> NAD+), donation of electron?
Glycerol Phosphate Dehydrogenase
What does the mitochondrial membrane version of Glycerol Phosphate Dehydrogenase do?
Converts Glycerol phosphate back into Dihydroxyacetone phosphate
- FAD -> FADH2
What does membranous Glycerol Phosphate Dehydrogenase produce / convert other than Dihydroxyacetone phosphate?
FADH2 (from FAD)
What is the disadvantage of the glycerol phosphate shuttle in comparison with the malate shuttle?
FADH2 (glycerol phosphate shuttle) cannot produce as many ATPs as NADH (which is produced from malate shuttle)
How does ETC generate energy?
Throigh extracting electrons from NADH and FADH2 to oxygen and in the process then generating energy (and water)
What is complex I also known as?
NADH Dehydrogenase
What does NADH dehydrogenase (complex I) do?
- Oxidises NADH (NADH -> NAD+)
- Transfers electrons (from NADH) to coenzyme Q (ubiquinone)
What is coenzyme Q also known as?
ubiquinone
What does CoQ shuttle electrons to?
Complex III
Where are the Electron transport complexes found?
On the inner membrane of the mitochondria
What is pumped into the inner membrane space by complex I?
H+ (later used to produce ATP)
What are CoQ10 supplements taken in conjunction with and why?
Statins
- Statins are thought to decrease CoQ levels
- No good data to support this
What is Complex II also known as?
Succinate dehydrogenase (TCA cycle)
How many electron transport complexes are there?
4
What are some of the key intermeadites of complex I?
- Flavin mononucleotide
- Iron sulfur compounds (FeS)
What does Complex II (Succinate dehydrogenase) transfer electrons to?
From Succinate (becomes fumarate)
- To FADH2
- Then to CoQ
What does succinate become as a result of complex II?
Fumarate
What is complex III also known as?
Cytochrome bc1 complex
What are the roles of complex III?
- Transfers electrons CoQ -> cytochrome c
- Pumps H+ to intermembrane space