Electromyography 1.2 - Measuring Neuromuscular Activity Flashcards

1
Q

the varying rates of MAP transmission may determine characteristics of the EMG signal. state and explain 3?

A

1) AP’s moving slow contribute to low-frequency components of surface EMG
2) as AP propagation is an ionic process, the AP conduction velocity along the muscle fibre is dependent on the rate at which these ions can be exchanged
3) the passive membrane part of the exchange rate, along with the active metabolic mechanism for pumping Na+ back out the fibre

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2
Q

state how (A) atrophied muscle fibres and (B) longer muscle fibres affect conduction velocity

A

both make it slower

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3
Q

how does superficial/deep location affect EMG

A

some temporal dispersion issues define the shape of the MUAP as measured by EMG –> deeper muscle fibres contribute less to the EMG signal

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4
Q

define - ‘the MU Discharge of Firing Rate’

A

the motor unit discharge of firing rate refers to the nervous system being able to control the frequency of Motor Unit recruitment

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5
Q

state, and explain, what it is meant by the key term - ‘Doublets’

A
  • motor units may also fire in two short latency bursts to overcome force before beginning a regular firing rate
  • Doublets (twin bursts) have the potential to produce more force than expected from the addition of two motor unit force twitches
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6
Q

state, and explain, the relationship between EMG amplitude and force

A

non-linear

moreover, compensatory activity of antagonist in agonist movement can occur so cannot assume that increases in EMG activity are indicative of parallel increases in force

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7
Q

Talk about ‘Monopolar Recordings’ of EMG (4 points)

A
  • one electrode on muscle belly & one on bony, inactive site
  • use din static contractions & clinical investigations (indwelling electrodes)
  • less stable & poor choice for non-isometric movements
  • are appropriate for assessments of H & T reflexes & M-waves
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8
Q

what is ‘Common Mode Rejection’ ? (2 points)

A
  • the feature that allows amplifiers to attenuate common signals
  • the common mode rejection ratio (CMRR) is expressed in a linear or logarithmic scale
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9
Q

care must be taken when setting an ‘Amplifier Gain’, but why? (4 points)

A
  • ‘clipping’ occurs when EMG signal is larger than amplifier gain set (amplifier is saturated)
  • clipping occurs when the amplitude of the output exceeds the bounds of the power supply
  • however, if gain is too low, resolution of the signal after Analogue-to-Digital (A-D_ conversion will be small
  • ideally, gain should be set so that the amplitude of the signal is matched to the range of the A/D converted
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10
Q

what technical issues arise with surface electrodes? (3 points)

A
  • usually, there is a 30 mV potential difference between inside and outside of skin layers
  • when the skin is stretched, the potential dec^ by approx 25 mV, resulting in a 5 mV artefact
  • these artefacts can be reduced with the use of silver electrodes & abraising the skin to reduce the usual 50 kΩ impedance across the skin surface
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11
Q

what method can be used which has been shown to successfully minimise artefacts ? (3 points)

A
  • locating the first-stage amplifier as close to the electrode as possible
  • friction & movement in cable movement can be reduced by using a gain operational amplifier at each electrode (termed active electrodes)
  • these electrodes position the amplifier very close to the sensor and therefore produce cleaner signals
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12
Q

what is the estimated limit of deepness of surface electrode readings?

A

10 - 20 mm (Barkhaus et al., 1994)

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13
Q

what else do surface electrodes have difficulty in measuring?

A

smaller muscles as difficult to say whether signal is coming from the smaller muscle or from adjacent muscles

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14
Q

what did Lexell et al., (1983) say about deeper motor units?

A

Lexell et al., (1983) provided some evidence that deeper motor units may be smaller than their superficial counterparts. Therefore, surface EMG recordings must be interpreted in the context of a possible bias toward recording from larger and more glycolytic motor units

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15
Q

what do fine wire electrodes facilitate?

A

fine-wire electrodes facilitate the recordings of deeper and smaller muscles

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16
Q

what is the configuration of fine-wire electrodes? (2 points)

A

their configuration generally consists of two fine diameter insulated wires that are threaded through a hollow needle cannula ]

the tips of the wires constitute the recording surfaces, and either these are cut flush of the last millimeter or so of insulation is removed from the wire

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17
Q

how are fine-wire electrodes usually used? (3 points)

A

the distal cm of these wires is bent backwards so that the wires and needle can be inserted into the muscle

after the needle is inserted, it is carefully removed, leaving just the wires as the recording electrodes

the cannula can either be removed or left off to the side during the experiment, and the ends of the wires are then connected to an amplifier

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18
Q

what are ‘Needle Electrodes’? (4 points)

A

1) frequently used to monitor activity of more individuals motor units rather than a whole muscle
2) concentric electrodes consist of a small wire placed in the middle of a hollow cannula
3) the wire is set in place with an epoxy
4) the cannula is then cut at an acute angle (approx. 15 degrees) leaving a shiny wire surface that is then referenced to the cannula for a bipolar recording

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19
Q

what are longitudinal array electordes?

A

used to record the features of propagating fibre AP’s

20
Q

what are multiple 2D array electrodes?

A

consist of 9 or more electrode surfaces arranged in a grid and are used to investigate the architectural features of muscle

21
Q

to record individual muscle fibre AP’s during high force contractions requires specialised electrodes…

A

can use multi-electrode arrays

but again, a surface detector is biased to superficial fibres

AP’s from individual muscle fibres are usually recorded from wire electrodes, multiple wire electrodes, or needle electrodes

22
Q

state what it is meant by the key term - Whole Muscle Mechanography (wMMG)

A

wMMG is a relatively new, non-invasive technique for determining muscle activity

23
Q

what does Whole Muscle Mechanography (wMMG) measure?

A

wMMG detects and measures the lateral displacement of a muscle belly’s following maximal percutaneous neuromuscular stimulation (PNS) via a needle that conducts an electrical current

24
Q

what is usually used to measure displacement in Whole Muscle Mechanography (wMMG)?

A

a laser sensor

the rise and fall of the muscle belly correlates with the development of tension longitudinally with the muscle tendons

25
Q

what is a benefit of Whole Muscle Mechanography (wMMG) ?

A

wMMG has growing evidence in its potential to estimate muscle fibre composition within a muscle (Djordjevic et al., 2001)

26
Q

state what it is meant by the key term - Near InfraRed Spectroscopy (NIRS)

A

NIRS is another non-invasive way to measure muscle activity, essentially by determining oxygenation levels within a muscle

27
Q

how does Near InfraRed Spectroscopy (NIRS) determine oxygenation levels within a muscle

A

light wavelengths between 600 - 1000 nm can penetrate the skin, bone, fat, and connective tissue, where it is either absorbed or shattered. this permits the determination of changes in blood volume as well as changes in the concentration of oxyhemoglobin

28
Q

What is the usual method of near InfraRed Spectroscopy (NIRS)?

(3 points)

A

1) typically uses a minimum of two light probes with different wavelengths
2) the longer wavelength is equally absorbed by oxygenated and deoxygenated Hb whereas short wavelength is primarily absorbed by deoxygenated Hb
3) the absorption difference between the two probes in then used in an index of skeletal muscle oxygen consumption

29
Q

state 3 benefits of Near InfraRed Spectroscopy (NIRS)

A

inexpensive

portable

the wavelengths can also penetrate the skull, so can be used to assess brain activity

30
Q

state, and explain, 2 disadvantages over the use of Near InfraRed Spectroscopy (NIRS)

A

1) light probes cannot penetrate very deeply into the brain before the light is scattered and the path-length of a given wavelength of light is not well known for different types of tissue
2) NIRS is also limited to providing information about the outermost layer of the brain –> the cortex

31
Q

what does Electroencephalography (EEG) involve?

4 points

A
  • placing several electrodes on scalp
  • measures voltage fluctuations in brain neurones beneath electrodes
  • 4 - 16 electrodes usually used
  • placement is characterised by lobe location (although no ‘central lobe’ - a central location is designated for identification purposes)
32
Q

how is EEG info transmitted?

A
  • EEG info is transmitted by wires to amplifiers and recording devises
  • as brain activity is rhythmic, the EEG recordings relate to specific rhythms
33
Q

what are the 4 brain waves in order of fastest to slowest

A

Beta Waves: occur when an area of the cortex is active

Alpha Waves: occur during quiet, awake status

Theta Waves: occur during some sleep states

Delta Waves: characteristic of deep sleep
- dreaming generates theta waves whereas non-dreaming sleep and comas generate delta waves

34
Q

Neural centres responsible for movement can be divided into 4 distinct categories. What is the first one? (3 points)

A

1st –> located in grey matter of spinal cord & tegmenjtum of brainstem

movements conveyed to muscles via lower motor neurones

provide coordination of muscles to create movement

35
Q

Neural centres responsible for movement can be divided into 4 distinct categories. What is the second one?

A

consists of upper motor neurones, cell bodies in brainstem & cortex - synapses descend onto lower circuit neuroes

essential for initiation of voluntary movements & spatio-temporal sequences of skilled movements

include Broca’s area, Broadman’s area…

36
Q

what is the function of the Broadman’s area?

A

governs the expression of expressions, especially with respect to fascial musculature

37
Q

what is the function of the Broca’s area?

A

the posterior portion of the frontal gyrus focused on the development of speech

38
Q

Neural centres responsible for movement can be divided into 4 distinct categories. What is the third and fourth ones?

A

massive, complex neural circuits

control movement indirectly by regulating the activity of upper motor neurones in the cerebral cortex & brainstem

39
Q

what is the function of the cerebellum

A

0verlies pons & 4th ventricle in posterior cranium

functions via afferent pathways to upper motor neurones as a servomechanism, detecting and attenuating the difference between intended and actual movements

mediate immediate and LT changes in error

40
Q

what do patients with cerebellum damage exhibit?

A

coordination - prestige errors in controlling the direction and amplitude of movements

41
Q

what is the function of the basal ganglia?

A

embedded in the depths of the forebrain

prevent upper motor neurones from initiating unwanted movements and prepare motor circuits for the initiation of movements

42
Q

how can the motor neurone-muscle relationship be examined

A
  • injecting muscles with specific tracers which are transported by their specific axons
43
Q

where are motor neurones innervating axial muscles located ?

A

in the ventral horn of the spinal cord

44
Q

where are motor neurones innervating shoulders located ?

A

lateral to the axial muscles motor neurone location

45
Q

where are lower motor control neurones innervating proximal arm muscles located ?

A

more lateral than shoulder motor neurones

46
Q

where are the motor neurones controlling distal arm muscles located

A

the most lateral from the middle of the spinal cord

47
Q

what is the reason for the spatial orientation of the MN pools? (3 points)

A

provides framework for understanding how descending projections of upper and intersegmental MN’s control posture & modulate movement

posture - long pathways in medial & anterior white matter of spinal cord

most lateral MN’s concerned with execution of skilled behaviour run through lateral white matter of the spinal cord