elastin stain

Question 1

what is elastin fibres

Answer 1

thinner than collagen fibres and are capable of stretching 1 and half time their length in return

Question 2

elastin fibres are found in

Answer 2

dermis, elastin cartilage, in blood vessels, ligaments, and lung

Question 3

why is elastin examined

Answer 3

there are some rare diseases which attack elastin fibres, or are caused by an abundance of elastin fibres

Question 4

what are two elastin stains

Answer 4

verhoeff’s iron hematoxylin
gomori’s aldehyde fuchsin

Question 5

verhoeff method

Answer 5

regressive stain of modified iron hematoxylin
elastin has a very strong affinity for the dye lake

Question 6

van geison counterstain

Answer 6

mix of picric acid and acid fuchsin
- controlled dye copetition and ionic attraction
- small molecule accesses all binding sites first, but acid fuchsin will remove it once it arrives

Question 7

verhoeff’s method notes

Answer 7

• ferric chloride is the oxidant, the mordant, and the differtiator
• picric acid differentiates slightly
• water wash after differentiation must be thorough
• suitable for course elastic fibres

Question 8

gomoris aldehyde fuchsin theory

Answer 8

used a single reagent and green counterstain
stains specifically for elastin