connective tissue stains Flashcards
what are the three main types of connective tissue fibres
collagen
reticulin
elastin
collagen fibres are produces by
fibroblast
what are the four types of collagen fibres
coarse, white, bifringent, longitudinally striated
collagen have many basic groups that are…
acidophillic
what is the purpose of the trichrome stains
demonstrate collagen distinctly
- required to show the extent of fibrosis or identify collagenous tumors
theory of trichrome methods
*** controlled dye competition
- stain nuclei black/dark first
- other components = contrasting
what is the parameters of the competition
areas with the smallest pore sized are hardest to access
- the bigger the dye molecule, the longer it takes to squeeze through
true or false: areas with the largest pore sized are easily permeability to all sizes of dyes
true: larger molecules displace smaller molecules
what is the order of major components (smallest to larger)
red blood cells
muscle
collagen
masson’s trichrome theory
selectively stain connective tissue with anionic dyes
- dye competition used to achieve contrast
what are the dye components of masson’s trichrome smallest to largest
- picric acid
- acid fuchsin
- phospholybdic/ phosphotungtic acid - large but binds very weakly
- light green or analine blue - intermediate size
is analine blue or light green used for small amounts of collagen
analine blue
what are used for control tissue for masson’s trichrome
lung, kidney, spleen, dermis
van gieson technique theory
section is stained with a solution of picric acid and acid fuchsin
what fixatives are used coincidingly with van gieson
mercuric chloride, dichromate, or picric acid
