ECOLI Flashcards

1
Q

Strain of Escherichia coli that causes infantile diarrhea?

A

Enteropathogenic E. coli (EPEC)

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2
Q

Which organism gives a mucoid colony under MacConkey Agar?

A

Klebsiella pneumoniae

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3
Q

It produces swarming motility and has a burnt chocolate odor.

A

Proteus spp.

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4
Q

All of the following genera are non-motile members of the family Enterobacteriaceae, except?

A

Proteus (motile, unlike Yersinia, Shigella, and Klebsiella)

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5
Q

What distinguishes Enteropathogenic E. coli (EPEC) from Enterohemorrhagic E. coli (EHEC)?

A

EHEC can cause hemolytic uremic syndrome (HUS).

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6
Q

What is the main pathogenic feature of Salmonella Typhi?

A

It invades and replicates within macrophages.

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7
Q

Produces a red pigment and gives a positive result for DNase.

A

Serratia marcescens

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8
Q

Which of the following is not H₂S positive?

A

Shigella dysenteriae

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9
Q

The causative agent of Bubonic Plague is?

A

Yersinia pestis

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10
Q

Which agar is highly selective for the isolation of Salmonella spp.?

A

HE agar (Hektoen Enteric Agar)

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11
Q

What organism is often associated with lobar pneumonia in elderly hospitalized patients?

A

Klebsiella pneumoniae

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12
Q

Which species produce a ‘stalactite pattern’ in broth cultures?

A

Yersinia pestis

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13
Q

What is a key distinguishing feature of Enterobacter species compared to E. coli?

A

Enterobacter is lactose-positive but indole-negative.

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14
Q

All of the organisms under Enterobacteriaceae are considered oxidase-negative except:

A

Plesiomonas shigelloides

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15
Q

An enteric organism that is acquired by eating improperly prepared and cooked or preserved food contaminated with human feces and produces dysentery?

A

Shigella spp.

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16
Q

Which of the following produces a ‘safety pin appearance’ on bipolar staining?

17
Q

A pathogen documented as the cause of nursery outbreaks of neonatal meningitis and brain abscesses.

A

Citrobacter koseri

18
Q

Why does Proteus mirabilis cause kidney stones?

A

It produces urease, raising urine pH and leading to calcium precipitation.

19
Q

Which of the following is a selective medium for the isolation of Yersinia enterocolitica?

20
Q

It may cause acute gastroenteritis or food poisoning.

A

Salmonella

21
Q

What is the carbohydrate source of XLD Agar?

22
Q

All of the following sugars are detected in Triple Sugar Iron (TSI) Agar except:

23
Q

The additive that is added to the OF test for differentiating oxidation and fermentation?

A

Mineral oil

24
Q

The end product of lysine decarboxylation is?

A

Cadaverine

25
Which of the following antigens is considered heat-stable?
Somatic (O) Antigen
26
The positive result of the citrate test?
Blue
27
It determines the ability of an organism to produce β-D-glucuronidase.
MUG Test
28
All of the following will give a positive result for the Urease Test, except:
Escherichia coli
29
How many drops of Methyl Red are required for every 5mL of broth when detecting the end products of glucose fermentation?
5-6 drops
30
For Decarboxylase and Dihydrolase Test, a positive result is indicated by what color?
Purple
31
A pink to wine-colored ring after the addition of Kovac’s reagent is a positive result for:
Indole Test
32
The pH indicator of Hektoen Enteric (HE) Agar is?
Bromthymol blue
33
Blackening of the medium indicates the presence of what substance?
Hydrogen sulfide (H₂S)
34
When glucose is fermented, the butt of the medium becomes acidic and will turn what color in LIA?
Yellow