ECM

Question 1

• Components:
  1. ) GAG: Structure? Usually attached to? Via? Called? 2 examples? Functions? (4)
  2. ) Collagen: Acts as? Structure? Rich in? Cross linked enzymes form?
  3. ) Fibronectin/laminin: Contain? Bind to? (4) Laminin almost exclusively in?
  4. ) Water/Solutes:
• Fibrillar proteins? (2)
• Multidoain adapter porteins? (2)
• MMP’s: Remodel ECM how? Functions? (2) Used by what as well?

Answer 1

1. ) polysaccharide with repeating disaccharide; protein; serine residue; proteoglycan; heparin and chondrioton; scaffold; bind proteins; bind GF’s; leukocytes bind it when rolling
2. ) Flexible structure; trimer; rich in glyceine; meshwork
3. ) Multisignalling domains; eachother, GAG, collagen and other cells; basement membrane
   - collagen and elastin
   - fibronectin and laminin
   - eating up matrix; allow cell migration and facilitate cell signalling; pathogens

Question 2

• Metazoans need what? (2)
  CAMS:
  1.) Cadherins: Structure? Bind what? Connect to within cytosol? (3) Which connect them to what?
  2.) Ig-CAMS: Similar to? But? Outside forms? No? Often found where? What cross links CAM to cytoskeleton? Often involved in?
  3.) Integrins: Structure? Forms? Important what? Related to? Connected to cell via? (3)
• Cancer cells lose what? How?

Answer 2

• Adherence and jenctions for communication
  1. ) Dimerized transmembrane protein; calcium; P120, a and B catenins; actin
  2. ) Cadherins; no/little cytoplasmic domain; Calcium; NS; Ankyrin; cell signalling within cell
  3. ) heterodimer with a and B sub units; dimer which forms matrix binding site; signal pathway; motility; actinin, talkin, filamin
• Integrin dependent survival; mutating part of cascade that would lead to apoptosis