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SOPs > E-0017 - Quanti-Tray (QT) > Flashcards

E-0017 - Quanti-Tray (QT) Flashcards

1
Q

What is the code for QT?

A

SM 9223 B-b

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2
Q

What is the first thing you do?

A

Make sure I have proper PPE on
- Lab coat, goggles, and gloves

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3
Q

What do you do after putting on PPE?

A

Turn on the Quanti-tray sealer machine
- Warm up for 10-15 minutes
- Ready when green light is on

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4
Q

What do you do after turning on the Quanti-tray sealer machine?

A

Prepare the logbook

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5
Q

Preparing the logbook: what information do you include?

A
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6
Q

What do you do after preparing the logbook?

A

Sterilize the work bench area, equipment, and gloves with 70% IPA

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7
Q

Describe the set up for a QT.

A
  1. In rack:
    - 3 QC bottles at the top: EC, KP, and PA
    - Samples along the bottom, left to right, 5 in a row
  2. Paper towels
    - Lay media packs on them, plastic side up
  3. Take out QT packets and label them with the sample description on the back
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8
Q

What do you do after sterilizing work area/equipment/gloves?

A

Check sample volume (should be 100 mL)
- If exceeds: pipette off excess
- If less than 95 mL: tell PM immediately
- If overfilled: transfer to sterile container to homogenize and transfer back to sample bottle

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9
Q

What must you do before pipetting off excess sample volume?

A

Shake the sample 25 times

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10
Q

What do you do after checking the sample volumes?

A

Acquire the appropriate media

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11
Q

How do you know what media you use?

A

Check the COC to see if the sample matrix is wastewater, storm water, drinking water, or solid

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12
Q

What media is used for wastewater and storm water?

A

Colilert 18 or Colilert 24 for TC and EC
- Only use Colisure if there is a tint

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13
Q

What media is used for drinking water?

A

Colisure, Colilert 18, or Colilert 24

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14
Q

What media is used for solids?

A

Do not use QT for solids

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15
Q

What media is used for wastewater (fecal coliforms)?

A

Only Colilert 18

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16
Q

What should you do if the samples need fecal coliform reported?

A

Make sure there are 2 containers (the incubation temperature for fecal is different)
- Will also need a separate batch of QC

17
Q

What do you do after acquiring the appropriate media?

A

Spray the front of packet (plastic side) with 70% IPA

18
Q

What do you do after sterilizing the media packets with 70% IPA?

A

Add the media to the samples and QC
- Gently swirl and invert to dissolve

19
Q

What do you do after adding the media to the samples?

A

Pour the samples into the corresponding packets
- Tap the wells to release the air bubbles

20
Q

What do you do after pouring the samples into the packets?

A

Run the packets through the sealer
- Place packet into rubber insert
- Run tray through the sealer
- Check for leaks after
- Sterilize the rubber insert after and between packets

21
Q

What do you do after sealing the trays?

A

Incubate the samples and QCs

22
Q

What is the incubation temperature for TC and EC?

A

35 +/- 0.5 degrees C

23
Q

What is the water bath temperature for fecal coliforms?

A

44.5 degrees C

24
Q

How long do you incubate Colisure and Colilert 24?

A

24-28 hours
- Inconclusive Colisure trays must be incubated for 48 hours

25
Q

How long do you incubate Colilert 18?

A

18-22 hours

26
Q

Are the trays incubated/water bath face up or face down?

A

Face down

27
Q

What do you do after putting the samples in the incubator?

A

Calculate the reading time based off the time you put it into the incubator or water bath
- Write on post-it and put in the middle of the page in the logbook

28
Q

Preparing the logbook for reading: what information do you include?

A
29
Q

After setting up the logbook for reading, what do you do?

A

Read the QCs first and record the results
- EC: color change and fluorescence
- KP: color change but not fluorescence
- PA: no color change and no fluorescence

30
Q

What do you do after reading the QCs?

A

Read the samples and record the results
- Count wells with color change: write number under “Total Coliform”
- Count wells with fluorescence: write number under “E. coli”

31
Q

What do you do after reading the samples and recording the results?

A
  • Cross out the post-it and move it to the bottom of the page
  • Cross out the to-do list
32
Q

What do you do after crossing out the post-it and to-do list?

A

Put the samples and QCs on the 24-hour hold shelf

33
Q

List the general steps of a QT (not including reading).

A
  1. PPE and get samples
  2. Turn on QT sealer machine
  3. Prep logbook
  4. Sterilize work area
  5. Check sample volume
  6. Sterilize media packets and add media
  7. Prep QCs
  8. Label QT packets
  9. Pour samples into packets
  10. Seal packets
  11. Incubate
  12. Calculate reading time
  13. Sterilize work area

SOPs (8 decks)

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