E-0016 - Enterolert

Question 1

What is the code for Enterolert?

Answer 1

SM 9230 D

Question 2

What is the fist thing you do?

Answer 2

Make sure I have proper PPE on
- Lab coat, goggles, and gloves

Question 3

What do you do after putting on PPE?

Answer 3

Turn on the Quanti-tray sealer machine
- Warm up for 10-15 minutes
- Ready when green light is on

Question 4

What do you do after turning on the Quanti-tray sealer machine?

Answer 4

Prepare the logbook

Question 5

Preparing the logbook: what information do you include?

Answer 5

Question 6

What do you do after preparing the logbook?

Answer 6

Sterilize the work bench area, equipment, and gloves with 70% IPA

Question 7

Describe the setup for an Enterolert.

Answer 7

1. In rack:
   - 2 QC bottles at the top: EF and EC
   - Samples along the bottom, left to right, 5 in a row
2. Paper towels
   - Lay media packs on them, plastic side up
3. Take out QT packets and label them with the sample description on the back

Question 8

What do you do after sterilizing work area/equipment/gloves?

Answer 8

Check sample volume (should be 100 mL)
- If exceeds: pipette off excess
- If less than 95 mL: tell PM immediately
- If overfilled: transfer to sterile container to homogenize and transfer back to sample bottle

Question 9

What must you do before pipetting off excess sample volume?

Answer 9

Shake the sample 25 times

Question 10

What do you do after checking the sample volumes?

Answer 10

Get Enterolert media

Question 11

What do you do after getting Enterolert media?

Answer 11

Spray the front of packet (plastic side) with 70% IPA

Question 12

What do you do after sterilizing the media packets with 70% IPA?

Answer 12

Add the media to the samples and QC
- Gently swirl and invert to dissolve

Question 13

What do you do after adding the media to the samples?

Answer 13

Pour the samples into the corresponding packets
- Tap the wells to release the air bubbles

Question 14

What do you do after pouring the samples into the packets?

Answer 14

Run the packets through the sealer
- Place packet into rubber insert
- Run tray through the sealer
- Check for leaks after
- Sterilize the rubber insert after and between packets

Question 15

What do you do after sealing the trays?

Answer 15

Incubate the samples and QCs

Question 16

What is the incubation temperature and time?

Answer 16

41 +/- 0.5°C (use Incubator B)

Question 17

What do you do after putting the samples in the incubator?

Answer 17

Calculate the reading time based off the time you put it into the incubator
- Write on post-it and put in the middle of the page in the logbook