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SOPs > E-0015 - SimPlate > Flashcards

E-0015 - SimPlate Flashcards

1
Q

What is the code for SimPlate?

A

SM 9215-E

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2
Q

What is the first thing you do?

A

Make sure I have proper PPE on
- Lab coat, goggles, and gloves

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3
Q

What do you do after putting on PPE?

A

Turn off the AC and prepare the logbook

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4
Q

Preparing the logbook: what information do you include?

A
  • Prep analyst initials
  • Prep date/time
  • Culture ID
  • Media lot #
  • Pipette lot #
  • Plate lot #
  • Sample names and numbers under “comments”
  • DF (dilution factor): 1X (no dilution)
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5
Q

What do you do before setting up an HPC?

A

Turn off the AC

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6
Q

What do you do after turning off the AC?

A

Sterilize the work bench area, equipment, and gloves with 70% IPA

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7
Q

What do you do after sterilizing your work area?

A

Check the sample volume (100 mL)

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8
Q

Describe the setup for a SimPlate.

A
  1. Line up sample bottles in order
  2. Line up air plate, media plate, and positive control in order
  3. Put two plates in front of each sample bottle (+2 for dups)
  4. Label each plate accordingly
  5. Open the air plate (record the air late open date/time)
  6. Have the media ready
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9
Q

What do you do after checking the sample volume?

A

Label the plates

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10
Q

What do you do after labeling the plates?

A

Open the air plate
- Record the air plate open date/time in the logbook

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11
Q

What do you do after opening the air plate?

A

Pipette 1.0 mL of sample onto each plate

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12
Q

What must you do before pipetting the samples on the plates?

A

Shake each sample 25 times

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13
Q

How much sample do you pipette onto each plate?

A

Pipette 1mL of sample onto the center of each plate

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14
Q

What media do you use?

A

Idexx SimPate rehydrated media

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15
Q

How much media do you pipette onto each plate?

A

10mL

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16
Q

What do you do after pipetting the samples onto the plates?

A

Pipette 9.0 mL of media onto the plates

17
Q

What do you do after pipetting the media onto the plates?

A

Gently swirl each plate

18
Q

What things should you keep in mind when swirling the plates?

A
  • Make sure media gets into each well
  • Don’t let too much media get absorbed
19
Q

What do you do after swirling each plate?

A

Tip the plate to drain the excess media into the absorbent pad

20
Q

What do you do after absorbing the extra media?

A

Prepare the QCs

21
Q

How do you prepare the QCs?

A
  1. Media plate
    - Pipette 10 mL of agar onto the plate
  2. Positive plate
    - Pipette 10 mL of agar onto the plate
    - Inoculate the agar with E. coli
  3. Air plate
    - You opened this before you started preparing your samples
    - After 15 minutes: add media and close the plate
22
Q

What do you do after preparing the QCs?

A

Invert the plates and incubate at 35 +/- 0.5 degrees C for 48 +/- 3 hours
- Record the incubator in time/temp
- Record the incubator ID

23
Q

What do you do after putting the samples in the incubator?

A

Calculate the read date/time
- Put post-it in the middle of the page

24
Q

Preparing the logbook for reading: what information do you include?

A
  • Read analyst initials
  • Read date/time
  • Incubator out time/temp
25
Q

After setting up the logbook for reading, what do you do?

A

Count the fluorescent wells in the QCs

26
Q

What do you do after reading the QCs?

A

Count the fluorescent wells in the samples

27
Q

What do you do after reading the samples?

A

Average the two counts obtained for each sample and dups
- Record in logbook under “Average Plate Count”
- Units: CFU

28
Q

What do you do after reading the plates and recording the results?

A
  • Cross out the post-it and move it to the bottom of the page
  • Cross out the to-do list
29
Q

What do you do after crossing out the post-it and to-do list?

A

Put the samples and QCs on the 24-hour hold shelf

30
Q

List the general steps for an HPC (not including reading).

A
  1. PPE
  2. Get samples
  3. Logbook
  4. Turn off AC
  5. Sterilize area
  6. Pipette samples (1.0 mL)
  7. Pipette media (10 mL)
  8. Prep QCs
  9. Incubate
  10. Reading time

SOPs (8 decks)

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