Dr Moir - Done

Question 1

What is CAPZ?

Answer 1

Capping protein at the barbed end of actin

Question 2

What is tropomodulin?

Answer 2

Capping protein at the pointed end of actin

Question 3

Which of the 3 types of actin is found in the muscle tissue?

Answer 3

alpha

Question 4

Of F and G actin, which is favoured under cellular conditions?

Answer 4

F

Question 5

What is the role of profilin?

Answer 5

allows ATP binding and therefore promotes polymerisation

Question 6

What is the role of Thymosin Beta 4?

Answer 6

binds to G actin pointed end preventing polymerisation

Question 7

What type of mutations affect dystrophin the most?

Answer 7

Deletion or misense as they affect the length

Question 8

What is the role of dystrophin?

Answer 8

Provides cellular structure

Question 9

What is the role of fimbrin?

Answer 9

link actin filaments

Question 10

where is fimbrin most commonly found?

Answer 10

microvilli and actin cables

Question 11

What is the role of filamin?

Answer 11

allows gel formation with cross linking of strands

Question 12

What is the role of Gelsolin?

Answer 12

Solubilises gels by causing a break and ATP hydrolysis

Question 13

What angle does Arp2/3 bind to actin at?

Answer 13

70 degrees

Question 14

Why is actin branched by MTs not?

Answer 14

actin has Arp2/3 which can bind allowing branching

Question 15

What is released in the muscle cell to allow the mysoin binding?

Answer 15

Ca2+ ions

Question 16

What is the name of the mysoin binding site?

Answer 16

tropomyosin

Question 17

Which part of the myosin is conserved?

Answer 17

head group

Question 18

What is the term used for the structure of the Myosin II tail domain?

Answer 18

coiled-coil

Question 19

What interaction is responsible for the coiled coil structure?

Answer 19

hydrophobic

Question 20

What type of myosin is present in muscle cells?

Answer 20

mysoin II

Question 21

what is the sarcomere?

Answer 21

basic unit of the myofibril which is in the muscle cell

Question 22

What is a stable Microtubule?

Answer 22

an MT which is stable in structure and is constantly present in non-differniating cells

Question 23

What is a transient MT?

Answer 23

a MT which is only stable for short periods of time and constantly changes depending on the stage in the cell cycle

Question 24

What is a heterodimer?

Answer 24

a tubulin monomer which consists of 2 domains; alpha and beta

Question 25

What is the main difference between alpha and beta tubulin?

Answer 25

alpha binds GTP

beta binds GDP

Question 26

What drug targets tubulin and which form?

Answer 26

taxol targets beta tubulin and is an anti cancer drug

Question 27

How many protofilaments make up a MT?

Answer 27

13

Question 28

What is the diameter of a MT?

Answer 28

24nm

Question 29

What is the diameter of an actin filament?

Answer 29

8nm

Question 30

What causes depolymerisation of MTs?

Answer 30

Break down of GTP to GDP at the cap

Question 31

What is the role of kinesin?

Answer 31

directed delivery of new organelles

Question 32

what is the role of dynein?

Answer 32

directed delivery back to cell body for recycling of old organelles

Question 33

What is MAP2?

Answer 33

MT stabilising protein which results in loosely packed MTs

Question 34

What is Tau?

Answer 34

MT stabilising protein which results in tight packed MTS

Question 35

What is the target for Alzheimer’s disease treatment?

Answer 35

Tau

Question 36

What method of movement does Myosin use?

Answer 36

Hand over Hand